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Open AccessArticle

Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates

School of Biological Sciences, Washington State University, Pullman, WA 99164, USA
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2019, 8(4), 336;
Received: 25 January 2019 / Revised: 3 April 2019 / Accepted: 6 April 2019 / Published: 10 April 2019
(This article belongs to the Special Issue Molecular and Cellular Mechanisms of Stress Responses)
The turnip Brassica rapa has important economic value and represents a good model system to study gene function in crop plants. ERF/AP2 transcription factors are a major group of proteins that are often involved in regulating stress-responses and developmental programs. Some ERF/AP2 proteins are targets of CULLIN3-based E3 ligases that use BTB/POZ-MATH proteins as substrate receptors. These receptors bind the transcription factor and facilitate their ubiquitylation and subsequent degradation via the 26S proteasome. Here, we show tissue and stress-dependent expression patterns for three Brassica rapa ERF/AP2 proteins that are closely related to Arabidopsis thaliana AtRAP2.4. Cloning of the Brassica genes showed that the corresponding proteins can assemble with a BPM protein and CULLIN3, and that they are instable in a 26S proteasome dependent manner. This work demonstrates the conserved nature of the ERF/AP2-CULLIN3-based E3 ligase interplay, and represents a first step to analyze their function in a commercially relevant crop plant. View Full-Text
Keywords: RAP2.4; stress; proteasome; transcription; PEST RAP2.4; stress; proteasome; transcription; PEST
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Mooney, S.; Al-Saharin, R.; Choi, C.M.; Tucker, K.; Beathard, C.; Hellmann, H.A. Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates. Cells 2019, 8, 336.

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