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Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates

School of Biological Sciences, Washington State University, Pullman, WA 99164, USA
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2019, 8(4), 336; https://doi.org/10.3390/cells8040336
Received: 25 January 2019 / Revised: 3 April 2019 / Accepted: 6 April 2019 / Published: 10 April 2019
(This article belongs to the Special Issue Molecular and Cellular Mechanisms of Stress Responses)
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Abstract

The turnip Brassica rapa has important economic value and represents a good model system to study gene function in crop plants. ERF/AP2 transcription factors are a major group of proteins that are often involved in regulating stress-responses and developmental programs. Some ERF/AP2 proteins are targets of CULLIN3-based E3 ligases that use BTB/POZ-MATH proteins as substrate receptors. These receptors bind the transcription factor and facilitate their ubiquitylation and subsequent degradation via the 26S proteasome. Here, we show tissue and stress-dependent expression patterns for three Brassica rapa ERF/AP2 proteins that are closely related to Arabidopsis thaliana AtRAP2.4. Cloning of the Brassica genes showed that the corresponding proteins can assemble with a BPM protein and CULLIN3, and that they are instable in a 26S proteasome dependent manner. This work demonstrates the conserved nature of the ERF/AP2-CULLIN3-based E3 ligase interplay, and represents a first step to analyze their function in a commercially relevant crop plant. View Full-Text
Keywords: RAP2.4; stress; proteasome; transcription; PEST RAP2.4; stress; proteasome; transcription; PEST
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Mooney, S.; Al-Saharin, R.; Choi, C.M.; Tucker, K.; Beathard, C.; Hellmann, H.A. Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates. Cells 2019, 8, 336.

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