Next Article in Journal
Strategies to Circumvent Host Innate Immune Response by Hepatitis C Virus
Next Article in Special Issue
Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS
Previous Article in Journal
C-Met as a Key Factor Responsible for Sustaining Undifferentiated Phenotype and Therapy Resistance in Renal Carcinomas
Article

N-Glycomic and Transcriptomic Changes Associated with CDX1 mRNA Expression in Colorectal Cancer Cell Lines

1
Center for Proteomics and Metabolomics, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
2
Cancer and Immunogenetics Laboratory, Weatherall Institute of Molecular Medicine, Department of Oncology, University of Oxford, Oxford OX3 9DS, UK
3
Institut de Pharmacologie et de Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, 31077 Toulouse, France
*
Authors to whom correspondence should be addressed.
Cells 2019, 8(3), 273; https://doi.org/10.3390/cells8030273
Received: 3 March 2019 / Revised: 13 March 2019 / Accepted: 18 March 2019 / Published: 22 March 2019
(This article belongs to the Special Issue Functional Glycomics)
The caudal-related homeobox protein 1 (CDX1) is a transcription factor, which is important in the development, differentiation, and homeostasis of the gut. Although the involvement of CDX genes in the regulation of the expression levels of a few glycosyltransferases has been shown, associations between glycosylation phenotypes and CDX1 mRNA expression have hitherto not been well studied. Triggered by our previous study, we here characterized the N-glycomic phenotype of 16 colon cancer cell lines, selected for their differential CDX1 mRNA expression levels. We found that high CDX1 mRNA expression associated with a higher degree of multi-fucosylation on N-glycans, which is in line with our previous results and was supported by up-regulated gene expression of fucosyltransferases involved in antenna fucosylation. Interestingly, hepatocyte nuclear factors (HNF)4A and HNF1A were, among others, positively associated with high CDX1 mRNA expression and have been previously proven to regulate antenna fucosylation. Besides fucosylation, we found that high CDX1 mRNA expression in cancer cell lines also associated with low levels of sialylation and galactosylation and high levels of bisection on N-glycans. Altogether, our data highlight a possible role of CDX1 in altering the N-glycosylation of colorectal cancer cells, which is a hallmark of tumor development. View Full-Text
Keywords: colorectal cancer cell lines; N-glycosylation; fucosylation; caudal-related homeobox protein 1 (CDX1), differentiation; fucosyltransferase (FUT), hepatocyte nuclear factor (HNF)4A; HNF1A colorectal cancer cell lines; N-glycosylation; fucosylation; caudal-related homeobox protein 1 (CDX1), differentiation; fucosyltransferase (FUT), hepatocyte nuclear factor (HNF)4A; HNF1A
Show Figures

Figure 1

MDPI and ACS Style

Holst, S.; Wilding, J.L.; Koprowska, K.; Rombouts, Y.; Wuhrer, M. N-Glycomic and Transcriptomic Changes Associated with CDX1 mRNA Expression in Colorectal Cancer Cell Lines. Cells 2019, 8, 273. https://doi.org/10.3390/cells8030273

AMA Style

Holst S, Wilding JL, Koprowska K, Rombouts Y, Wuhrer M. N-Glycomic and Transcriptomic Changes Associated with CDX1 mRNA Expression in Colorectal Cancer Cell Lines. Cells. 2019; 8(3):273. https://doi.org/10.3390/cells8030273

Chicago/Turabian Style

Holst, Stephanie, Jennifer L. Wilding, Kamila Koprowska, Yoann Rombouts, and Manfred Wuhrer. 2019. "N-Glycomic and Transcriptomic Changes Associated with CDX1 mRNA Expression in Colorectal Cancer Cell Lines" Cells 8, no. 3: 273. https://doi.org/10.3390/cells8030273

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop