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Open AccessArticle

Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS

Graduate School of Analytical Science & Technology, Chungnam National University, Daejeon 34134, Korea
Asia-Pacific Glycomics Reference Site, Daejeon 34134, Korea
Research Center of Bioconvergence Analysis, Korea Basic Science Institute, Cheongju-si 28119, Korea
Department of Cancer Biomedical Science, National Cancer Center Graduate School of Cancer Science and Policy, Goyang 10408, Korea
Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong-gu, Daejeon 34141, Korea
Department of Biomolecular Science, KRIBB School of Bioscience, Korea University of Science and Technology (UST), Yuseong-gu, Daejeon 34113, Korea
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2019, 8(11), 1323;
Received: 30 September 2019 / Revised: 20 October 2019 / Accepted: 23 October 2019 / Published: 26 October 2019
(This article belongs to the Special Issue Functional Glycomics)
Gangliosides act as a surface marker at the outer cellular membrane and play key roles in cancer cell invasion and metastasis. Despite the biological importance of gangliosides, they have been still poorly characterized due to the lack of effective analytical tools. Herein, we performed molecular profiling and structural elucidation of intact gangliosides in various cell lines including CFPAC1, A549, NCI-H358, MCF7, and Caski. We identified and quantified a total of 76 gangliosides on cell membrane using C18 LC-MS/MS. Gangliosides found in each cell line exhibited high complexity and diversity both qualitatively and quantitatively. The most abundant species was GM3(d34:1) in CFPAC1, NCI-H358, and MCF7, while GM2(d34:1) and GM1(d34:1) were major components in A549 and Caski, respectively. Notably, glycan moieties showed more diversity between cancer cell lines than ceramide moieties. In addition, noncancerous pancreatic cell line (hTERT/HPNE) could be distinguished by gangliosides containing different levels of sialic acid compared with cancerous pancreatic cell line (CFPAC1). These results clearly demonstrated the feasibility of our analytical platform to comprehensive profile of cell surface gangliosides for identifying cell types and subgrouping cancer cell types. View Full-Text
Keywords: glycosylation; ganglioside; cancer cell; cell surface; LC-MS/MS glycosylation; ganglioside; cancer cell; cell surface; LC-MS/MS
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Lee, J.; Hwang, H.; Kim, S.; Hwang, J.; Yoon, J.; Yin, D.; Choi, S.I.; Kim, Y.-H.; Kim, Y.-S.; An, H.J. Comprehensive Profiling of Surface Gangliosides Extracted from Various Cell Lines by LC-MS/MS. Cells 2019, 8, 1323.

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