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Open AccessArticle

Plant MIR167e-5p Inhibits Enterocyte Proliferation by Targeting β-Catenin

Guangdong Provincial Key Laboratory of Animal Nutritional Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Present Address: Yong-Liang Zhang, College of Animal Science, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
Cells 2019, 8(11), 1385; https://doi.org/10.3390/cells8111385
Received: 5 June 2019 / Revised: 11 July 2019 / Accepted: 31 October 2019 / Published: 4 November 2019
(This article belongs to the Special Issue Wnt Signaling in Health and Diseases)
MicroRNAs (miRNAs) are important negative regulators of genes involved in physiological and pathological processes in plants and animals. It is worth exploring whether plant miRNAs play a cross-kingdom regulatory role in animals. Herein, we found that plant MIR167e-5p regulates the proliferation of enterocytes in vitro. A porcine jejunum epithelial cell line (IPEC-J2) and a human colon carcinoma cell line (Caco-2) were treated with 0, 10, 20, and 40 pmol of synthetic 2′-O-methylated plant MIR167e-5p, followed by a treatment with 20 pmol of MIR167e-5p for 0, 24, 48, and 72 h. The cells were counted, and IPEC-J2 cell viability was determined by the MTT and EdU assays at different time points. The results showed that MIR167e-5p significantly inhibited the proliferation of enterocytes in a dose- and time-dependent manner. Bioinformatics prediction and a luciferase reporter assay indicated that MIR167e-5p targets β-catenin. In IPEC-J2 and Caco-2 cells, MIR167e-5p suppressed proliferation by downregulating β-catenin mRNA and protein levels. MIR167e-5p relieved this inhibition. Similar results were achieved for the β-catenin downstream target gene c-Myc and the proliferation-associated gene PCNA. This research demonstrates that plant MIR167e-5p can inhibit enterocyte proliferation by targeting the β-catenin pathway. More importantly, plant miRNAs may be a new class of bioactive molecules for epigenetic regulation in humans and animals. View Full-Text
Keywords: MIR167e-5p; β-catenin; enterocyte; proliferation MIR167e-5p; β-catenin; enterocyte; proliferation
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Li, M.; Chen, T.; He, J.-J.; Wu, J.-H.; Luo, J.-Y.; Ye, R.-S.; Xie, M.-Y.; Zhang, H.-J.; Zeng, B.; Liu, J.; Xi, Q.-Y.; Jiang, Q.-Y.; Sun, J.-J.; Zhang, Y.-L. Plant MIR167e-5p Inhibits Enterocyte Proliferation by Targeting β-Catenin. Cells 2019, 8, 1385.

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