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Open AccessArticle

Gγ and Gα Identity Dictate a G-Protein Heterotrimer Plasma Membrane Targeting

Department of Physical Biochemistry, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland
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Cells 2019, 8(10), 1246; https://doi.org/10.3390/cells8101246
Received: 17 September 2019 / Revised: 10 October 2019 / Accepted: 11 October 2019 / Published: 13 October 2019
(This article belongs to the Special Issue New Advances in Cyclic AMP Signalling)
Heterotrimeric G-proteins along with G-protein-coupled receptors (GPCRs) regulate many biochemical functions by relaying the information from the plasma membrane to the inside of the cell. The lipid modifications of Gα and Gγ subunits, together with the charged regions on the membrane interaction surface, provide a peculiar pattern for various heterotrimeric complexes. In a previous study, we found that Gαs and Gαi3 prefer different types of membrane-anchor and subclass-specific lipid domains. In the present report, we examine the role of distinct Gγ subunits in the membrane localization and spatiotemporal dynamics of Gαs and Gαi3 heterotrimers. We characterized lateral diffusion and G-protein subunit interactions in living cells using fluorescence recovery after photobleaching (FRAP) microscopy and fluorescence resonance energy transfer (FRET) detected by fluorescence lifetime imaging microscopy (FLIM), respectively. The interaction of Gγ subunits with specific lipids was confirmed, and thus the modulation of heterotrimeric G-protein localization. However, the Gα subunit also modulates trimer localization, and so the membrane distribution of heterotrimeric G-proteins is not dependent on Gγ only. View Full-Text
Keywords: protein-membrane interaction; spatiotemporal protein dynamics; G-proteins; FLIM-FRET; FRAP protein-membrane interaction; spatiotemporal protein dynamics; G-proteins; FLIM-FRET; FRAP
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MDPI and ACS Style

Mystek, P.; Rysiewicz, B.; Gregrowicz, J.; Dziedzicka-Wasylewska, M.; Polit, A. Gγ and Gα Identity Dictate a G-Protein Heterotrimer Plasma Membrane Targeting. Cells 2019, 8, 1246. https://doi.org/10.3390/cells8101246

AMA Style

Mystek P, Rysiewicz B, Gregrowicz J, Dziedzicka-Wasylewska M, Polit A. Gγ and Gα Identity Dictate a G-Protein Heterotrimer Plasma Membrane Targeting. Cells. 2019; 8(10):1246. https://doi.org/10.3390/cells8101246

Chicago/Turabian Style

Mystek, Paweł; Rysiewicz, Beata; Gregrowicz, Jan; Dziedzicka-Wasylewska, Marta; Polit, Agnieszka. 2019. "Gγ and Gα Identity Dictate a G-Protein Heterotrimer Plasma Membrane Targeting" Cells 8, no. 10: 1246. https://doi.org/10.3390/cells8101246

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