Next Article in Journal
Adoptive T Cell Therapy Strategies for Viral Infections in Patients Receiving Haematopoietic Stem Cell Transplantation
Next Article in Special Issue
Yeast to Study Human Purine Metabolism Diseases
Previous Article in Journal
HIF-1α and HIF-2α Differently Regulate the Radiation Sensitivity of NSCLC Cells
Previous Article in Special Issue
Dictyostelium: An Important Source of Structural and Functional Diversity in Drug Discovery
Review

Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium

Department of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
*
Author to whom correspondence should be addressed.
Cells 2019, 8(1), 46; https://doi.org/10.3390/cells8010046
Received: 15 December 2018 / Revised: 4 January 2019 / Accepted: 9 January 2019 / Published: 12 January 2019
In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including Dictyostelium, enabling rapid high-fidelity modification of endogenous genes. Here we reviewed recent progress in genome editing in Dictyostelium and summarised useful CRISPR vectors that express sgRNA and Cas9, including several microorganisms. Using these vectors, precise genome modifications can be achieved within 2–3 weeks, beginning with the design of the target sequence. Finally, we discussed future perspectives on the use of CRISPR/Cas9-mediated genome editing in Dictyostelium. View Full-Text
Keywords: CRISPR/Cas9; CRISPR vector; Dictyostelium; genome editing; tRNA-based expression; Amoebozoa CRISPR/Cas9; CRISPR vector; Dictyostelium; genome editing; tRNA-based expression; Amoebozoa
Show Figures

Figure 1

MDPI and ACS Style

Muramoto, T.; Iriki, H.; Watanabe, J.; Kawata, T. Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium. Cells 2019, 8, 46. https://doi.org/10.3390/cells8010046

AMA Style

Muramoto T, Iriki H, Watanabe J, Kawata T. Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium. Cells. 2019; 8(1):46. https://doi.org/10.3390/cells8010046

Chicago/Turabian Style

Muramoto, Tetsuya, Hoshie Iriki, Jun Watanabe, and Takefumi Kawata. 2019. "Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium" Cells 8, no. 1: 46. https://doi.org/10.3390/cells8010046

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop