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Dictyostelium: An Important Source of Structural and Functional Diversity in Drug Discovery
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Cells 2019, 8(1), 46; https://doi.org/10.3390/cells8010046

Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium

Department of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
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Received: 15 December 2018 / Revised: 4 January 2019 / Accepted: 9 January 2019 / Published: 12 January 2019
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Abstract

In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including Dictyostelium, enabling rapid high-fidelity modification of endogenous genes. Here we reviewed recent progress in genome editing in Dictyostelium and summarised useful CRISPR vectors that express sgRNA and Cas9, including several microorganisms. Using these vectors, precise genome modifications can be achieved within 2–3 weeks, beginning with the design of the target sequence. Finally, we discussed future perspectives on the use of CRISPR/Cas9-mediated genome editing in Dictyostelium. View Full-Text
Keywords: CRISPR/Cas9; CRISPR vector; Dictyostelium; genome editing; tRNA-based expression; Amoebozoa CRISPR/Cas9; CRISPR vector; Dictyostelium; genome editing; tRNA-based expression; Amoebozoa
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Muramoto, T.; Iriki, H.; Watanabe, J.; Kawata, T. Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium. Cells 2019, 8, 46.

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