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Cells 2018, 7(12), 227; https://doi.org/10.3390/cells7120227

Triple-Helical DNA in Drosophila Heterochromatin

Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo 05508-090, Brazil
Received: 2 November 2018 / Revised: 15 November 2018 / Accepted: 16 November 2018 / Published: 23 November 2018
(This article belongs to the Special Issue Nuclear Organisation)
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Abstract

Polynucleotide chains obeying Watson-Crick pairing are apt to form non-canonical complexes such as triple-helical nucleic acids. From early characterization in vitro, their occurrence in vivo has been strengthened by increasing evidence, although most remain circumstantial particularly for triplex DNA. Here, different approaches were employed to specify triple-stranded DNA sequences in the Drosophila melanogaster chromosomes. Antibodies to triplex nucleic acids, previously characterized, bind to centromeric regions of mitotic chromosomes and also to the polytene section 59E of mutant strains carrying the brown dominant allele, indicating that AAGAG tandem satellite repeats are triplex-forming sequences. The satellite probe hybridized to AAGAG-containing regions omitting chromosomal DNA denaturation, as expected, for the intra-molecular triplex DNA formation model in which single-stranded DNA coexists with triplexes. In addition, Thiazole Orange, previously described as capable of reproducing results obtained by antibodies to triple-helical DNA, binds to AAGAG repeats in situ thus validating both detection methods. Unusual phenotype and nuclear structure exhibited by Drosophila correlate with the non-canonical conformation of tandem satellite arrays. From the approaches that lead to the identification of triple-helical DNA in chromosomes, facilities particularly provided by Thiazole Orange use may broaden the investigation on the occurrence of triplex DNA in eukaryotic genomes. View Full-Text
Keywords: chromosomes; heterochromatin; Drosophila; triplex DNA; antibodies; thiazole orange; satellite repeats chromosomes; heterochromatin; Drosophila; triplex DNA; antibodies; thiazole orange; satellite repeats
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Gorab, E. Triple-Helical DNA in Drosophila Heterochromatin. Cells 2018, 7, 227.

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