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Cells 2018, 7(10), 144; https://doi.org/10.3390/cells7100144

Lipid Emulsion Inhibits the Late Apoptosis/Cardiotoxicity Induced by Doxorubicin in Rat Cardiomyoblasts

1
Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju-si 52727, Korea
2
Institute of Health Sciences, Gyeongsang National University, Jinju 52727, Korea
3
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Changwon Hospital, Changwon 51427, Korea
4
Department of Physiology, Gyeongsang National University School of Medicine, Jinju-si 52727, Korea
5
Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Jinju-si 52727, Korea
These authors contributed equally to this work.
Current address: Reproductive Physiology Lab, Animal Physiology Division, ICAR-NIANP, Adugodi, Bangalore-560030, India.
*
Author to whom correspondence should be addressed.
Received: 30 August 2018 / Revised: 9 September 2018 / Accepted: 18 September 2018 / Published: 20 September 2018
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Abstract

This study aimed to examine the effect of lipid emulsion on the cardiotoxicity induced by doxorubicin in H9c2 rat cardiomyoblasts and elucidates the associated cellular mechanism. The effects of lipid emulsion on cell viability, Bax, cleaved caspase-8, cleaved capase-3, Bcl-XL, apoptosis, reactive oxygen species (ROS), malondialdehyde, superoxide dismutase (SOD), catalase and mitochondrial membrane potential induced by doxorubicin were examined. Treatment with doxorubicin decreased cell viability, whereas pretreatment with lipid emulsion reduced the effect of doxorubicin by increasing cell viability. Lipid emulsion also suppressed the increased expression of cleaved caspase-3, cleaved caspase-8, and Bax induced by doxorubicin. Moreover, pretreatment with lipid emulsion decreased the increased Bax/Bcl-XL ratio induced by doxorubicin. Doxorubicin-induced late apoptosis was reduced by treatment with lipid emulsion. In addition, pretreatment with lipid emulsion prior to doxorubicin enhanced glycogen synthase kinase-3β phosphorylation. The increased malondialdehyde and ROS levels by doxorubicin were reduced by lipid emulsion pretreatment. Furthermore, lipid emulsion attenuated the reduced SOD and catalase activity and the decreased mitochondrial membrane potential induced by doxorubicin. Taken together, these results suggest that lipid emulsion attenuates doxorubicin-induced late apoptosis, which appears to be associated with the inhibition of oxidative stress induced by doxorubicin. View Full-Text
Keywords: lipid emulsion; doxorubicin; apoptosis; cardiotoxicity; cardiomyoblasts lipid emulsion; doxorubicin; apoptosis; cardiotoxicity; cardiomyoblasts
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Subbarao, R.B.; Ok, S.-H.; Lee, S.H.; Kang, D.; Kim, E.-J.; Kim, J.-Y.; Sohn, J.-T. Lipid Emulsion Inhibits the Late Apoptosis/Cardiotoxicity Induced by Doxorubicin in Rat Cardiomyoblasts. Cells 2018, 7, 144.

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