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Cells 2017, 6(3), 20;

Assays to Monitor Autophagy Progression in Cell Cultures

Department of Cell Biology, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands
Author to whom correspondence should be addressed.
Received: 5 June 2017 / Revised: 2 July 2017 / Accepted: 4 July 2017 / Published: 7 July 2017
(This article belongs to the Special Issue Assays to Monitor Autophagy in Model Systems)
Full-Text   |   PDF [243 KB, uploaded 7 July 2017]


The vast number of implications of autophagy in multiple areas of life sciences and medicine has attracted the interest of numerous scientists that aim to unveil the role of this process in specific physiological and pathological contexts. Cell cultures are one of the most frequently used experimental setup for the investigation of autophagy. As a result, it is essential to assess this highly regulated molecular pathway with efficient and reliable methods. Each method has its own advantages and disadvantages. Here, we present a review summarizing the most established assays used to monitor autophagy induction and progression in cell cultures, in order to guide researchers in the selection of the most optimal solution for their experimental setup and design. View Full-Text
Keywords: autophagy; autophagosome; phagophore; autophagic flux; measurement; assay; method autophagy; autophagosome; phagophore; autophagic flux; measurement; assay; method
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Orhon, I.; Reggiori, F. Assays to Monitor Autophagy Progression in Cell Cultures. Cells 2017, 6, 20.

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