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Cells
Volume 12
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10.3390/cells12182282
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Article

Complement C3 Reduces Apoptosis via Interaction with the Intrinsic Apoptotic Pathway

by
Zhou Fang
1,
Haekyung Lee
1,
Junying Liu
1,
Karen A. Wong
1,
Lewis M. Brown
2,
Xiang Li
1,
Alus M. Xiaoli
3,
Fajun Yang
3 and
Ming Zhang
1,4,*
1
Departments of Anesthesiology, SUNY Downstate Health Science University, 450 Clarkson Avenue, Brooklyn, NY 11203, USA
2
Quantitative Proteomics and Metabolomics Center, Department of Biological Sciences, Columbia University, New York, NY 10027, USA
3
Department of Medicine/Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
4
Departments of Cell Biology, SUNY Downstate Health Science University, 450 Clarkson Avenue, Brooklyn, NY 11203, USA
*
Author to whom correspondence should be addressed.
Cells 2023, 12(18), 2282; https://doi.org/10.3390/cells12182282
Submission received: 4 August 2023 / Revised: 7 September 2023 / Accepted: 11 September 2023 / Published: 15 September 2023
(This article belongs to the Collection Molecular and Cellular Mechanisms of Ischemia-Reperfusion Injury and Protective Strategies)
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Abstract

Myocardial ischemia/reperfusion (I/R) elicits an acute inflammatory response involving complement factors. Recently, we reported that myocardial necrosis was decreased in complement C3−/− mice after heart I/R. The current study used the same heart model to test the effect of C3 on myocardial apoptosis and investigated if C3 regulation of apoptosis occurred in human cardiomyocytes. Comparative proteomics analyses found that cytochrome c was present in the myocardial C3 complex of WT mice following I/R. Incubation of exogenous human C3 reduced apoptosis in a cell culture system of human cardiomyocytes that did not inherently express C3. In addition, human C3 inhibited the intrinsic apoptosis pathway in a cell-free apoptosis system. Finally, human pro-C3 was found to bind with an apoptotic factor, pro-caspase 3, in a cell-free system. Thus, we present firsthand evidence showing that C3 readily reduces myocardial apoptosis via interaction with the intrinsic apoptotic pathway.
Keywords: ischemia/reperfusion injury (IRI); complement C3; apoptosis; cytochrome c; pro-caspase 3 ischemia/reperfusion injury (IRI); complement C3; apoptosis; cytochrome c; pro-caspase 3

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MDPI and ACS Style

Fang, Z.; Lee, H.; Liu, J.; Wong, K.A.; Brown, L.M.; Li, X.; Xiaoli, A.M.; Yang, F.; Zhang, M. Complement C3 Reduces Apoptosis via Interaction with the Intrinsic Apoptotic Pathway. Cells 2023, 12, 2282. https://doi.org/10.3390/cells12182282

AMA Style

Fang Z, Lee H, Liu J, Wong KA, Brown LM, Li X, Xiaoli AM, Yang F, Zhang M. Complement C3 Reduces Apoptosis via Interaction with the Intrinsic Apoptotic Pathway. Cells. 2023; 12(18):2282. https://doi.org/10.3390/cells12182282

Chicago/Turabian Style

Fang, Zhou, Haekyung Lee, Junying Liu, Karen A. Wong, Lewis M. Brown, Xiang Li, Alus M. Xiaoli, Fajun Yang, and Ming Zhang. 2023. "Complement C3 Reduces Apoptosis via Interaction with the Intrinsic Apoptotic Pathway" Cells 12, no. 18: 2282. https://doi.org/10.3390/cells12182282

APA Style

Fang, Z., Lee, H., Liu, J., Wong, K. A., Brown, L. M., Li, X., Xiaoli, A. M., Yang, F., & Zhang, M. (2023). Complement C3 Reduces Apoptosis via Interaction with the Intrinsic Apoptotic Pathway. Cells, 12(18), 2282. https://doi.org/10.3390/cells12182282

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