Next Article in Journal
Rnd3 Is a Crucial Mediator of the Invasive Phenotype of Glioblastoma Cells Downstream of Receptor Tyrosine Kinase Signalling
Next Article in Special Issue
Reactive Oxygen and Nitrogen Species (RONS) and Cytokines—Myokines Involved in Glucose Uptake and Insulin Resistance in Skeletal Muscle
Previous Article in Journal
When the Gates Swing Open Only: Arrhythmia Mutations That Target the Fast Inactivation Gate of Nav1.5
Previous Article in Special Issue
Deficient Sarcolemma Repair in ALS: A Novel Mechanism with Therapeutic Potential
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Cells
Volume 11
Issue 23
10.3390/cells11233715
cells-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Redox Balance Differentially Affects Biomechanics in Permeabilized Single Muscle Fibres—Active and Passive Force Assessments with the Myorobot

by
Mena Michael
1,2,
Larisa Kovbasyuk
1,
Paul Ritter
1,2,
Michael B. Reid
3,
Oliver Friedrich
1,2 and
Michael Haug
1,2,*
1
Institute of Medical Biotechnology, Department of Chemical and Biological Engineering, Friedrich-Alexander-University Erlangen-Nürnberg, Paul-Gordan-Str. 3, 91052 Erlangen, Germany
2
Erlangen Graduate School in Advanced Optical Technologies (SAOT), Paul-Gordan-Str. 6, 91052 Erlangen, Germany
3
College of Health & Human Performance, University of Florida, 1864 Stadium Road, Gainesville, FL 32611, USA
*
Author to whom correspondence should be addressed.
Cells 2022, 11(23), 3715; https://doi.org/10.3390/cells11233715
Submission received: 26 August 2022 / Revised: 16 November 2022 / Accepted: 17 November 2022 / Published: 22 November 2022
(This article belongs to the Special Issue Redox Control of Cell Signaling in Cardiac and Skeletal Muscle)
Browse Figures
Versions Notes

Abstract

An oxidizing redox state imposes unique effects on the contractile properties of muscle. Permeabilized fibres show reduced active force generation in the presence of H2O2. However, our knowledge about the muscle fibre’s elasticity or flexibility is limited due to shortcomings in assessing the passive stress–strain properties, mostly due to technically limited experimental setups. The MyoRobot is an automated biomechatronics platform that is well-capable of not only investigating calcium responsiveness of active contraction but also features precise stretch actuation to examine the passive stress–strain behaviour. Both were carried out in a consecutive recording sequence on the same fibre for 10 single fibres in total. We denote a significantly diminished maximum calcium-saturated force for fibres exposed to ≥500 µM H2O2, with no marked alteration of the pCa50 value. In contrast to active contraction (e.g., maximum isometric force activation), passive restoration stress (force per area) significantly increases for fibres exposed to an oxidizing environment, as they showed a non-linear stress–strain relationship. Our data support the idea that a highly oxidizing environment promotes non-linear fibre stiffening and confirms that our MyoRobot platform is a suitable tool for investigating redox-related changes in muscle biomechanics.
Keywords: redox balance; single muscle fibre; calcium sensitivity; passive stiffness; MyoRobot redox balance; single muscle fibre; calcium sensitivity; passive stiffness; MyoRobot

Share and Cite

MDPI and ACS Style

Michael, M.; Kovbasyuk, L.; Ritter, P.; Reid, M.B.; Friedrich, O.; Haug, M. Redox Balance Differentially Affects Biomechanics in Permeabilized Single Muscle Fibres—Active and Passive Force Assessments with the Myorobot. Cells 2022, 11, 3715. https://doi.org/10.3390/cells11233715

AMA Style

Michael M, Kovbasyuk L, Ritter P, Reid MB, Friedrich O, Haug M. Redox Balance Differentially Affects Biomechanics in Permeabilized Single Muscle Fibres—Active and Passive Force Assessments with the Myorobot. Cells. 2022; 11(23):3715. https://doi.org/10.3390/cells11233715

Chicago/Turabian Style

Michael, Mena, Larisa Kovbasyuk, Paul Ritter, Michael B. Reid, Oliver Friedrich, and Michael Haug. 2022. "Redox Balance Differentially Affects Biomechanics in Permeabilized Single Muscle Fibres—Active and Passive Force Assessments with the Myorobot" Cells 11, no. 23: 3715. https://doi.org/10.3390/cells11233715

APA Style

Michael, M., Kovbasyuk, L., Ritter, P., Reid, M. B., Friedrich, O., & Haug, M. (2022). Redox Balance Differentially Affects Biomechanics in Permeabilized Single Muscle Fibres—Active and Passive Force Assessments with the Myorobot. Cells, 11(23), 3715. https://doi.org/10.3390/cells11233715

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop