Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish (Cyclopterus lumpus L.)

Sindre, Hilde; Gjessing, Mona C.; Fosse, Johanna Hol; Hermansen, Lene C.; Böckerman, Inger; Amundsen, Marit M.; Dahle, Maria K.; Solhaug, Anita

doi:10.3390/cells10092442

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Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish (Cyclopterus lumpus L.)

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Norwegian Veterinary Institute, 1433 Ås, Norway

Imaging Centre, Norwegian University of Life Sciences, 1432 Ås, Norway

Norwegian Institute of Public Health, 0213 Oslo, Norway

Author to whom correspondence should be addressed.

Academic Editor: Daniel Żarski

Cells 2021, 10(9), 2442; https://doi.org/10.3390/cells10092442

Received: 16 July 2021 / Revised: 19 August 2021 / Accepted: 4 September 2021 / Published: 16 September 2021

(This article belongs to the Section Cell Methods)

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Abstract

The use of lumpfish (Cyclopterus lumpus) as a cleaner fish to fight sea lice infestation in farmed Atlantic salmon has become increasingly common. Still, tools to increase our knowledge about lumpfish biology are lacking. Here, we successfully established and characterized the first Lumpfish Gill cell line (LG-1). LG-1 are adherent, homogenous and have a flat, stretched-out and almost transparent appearance. Transmission electron microscopy revealed cellular protrusions and desmosome-like structures that, together with their ability to generate a transcellular epithelial/endothelial resistance, suggest an epithelial or endothelial cell type. Furthermore, the cells exert Cytochrome P450 1A activity. LG-1 supported the propagation of several viruses that may lead to severe infectious diseases with high mortalities in fish farming, including viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). Altogether, our data indicate that the LG-1 cell line originates from an epithelial or endothelial cell type and will be a valuable in vitro research tool to study gill cell function as well as host-pathogen interactions in lumpfish. View Full-Text

Keywords: cleaner fish; lumpfish; respiration; epithelial; endothelial; virus; animal welfare; 3R; medical delousing; marine fish

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MDPI and ACS Style

Sindre, H.; Gjessing, M.C.; Fosse, J.H.; Hermansen, L.C.; Böckerman, I.; Amundsen, M.M.; Dahle, M.K.; Solhaug, A. Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish (Cyclopterus lumpus L.). Cells 2021, 10, 2442. https://doi.org/10.3390/cells10092442

AMA Style

Sindre H, Gjessing MC, Fosse JH, Hermansen LC, Böckerman I, Amundsen MM, Dahle MK, Solhaug A. Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish (Cyclopterus lumpus L.). Cells. 2021; 10(9):2442. https://doi.org/10.3390/cells10092442

Chicago/Turabian Style

Sindre, Hilde, Mona C. Gjessing, Johanna H. Fosse, Lene C. Hermansen, Inger Böckerman, Marit M. Amundsen, Maria K. Dahle, and Anita Solhaug. 2021. "Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish (Cyclopterus lumpus L.)" Cells 10, no. 9: 2442. https://doi.org/10.3390/cells10092442

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Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish (Cyclopterus lumpus L.)

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