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Comparison of a New 68Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction

1
Pharmacological Faculty, Aix Marseille University, INSERM 1263, INRAE 1260, C2VN, 13385 Marseille, France
2
Medical Faculty, Aix-Marseille University, CNRS 2012, CERIMED, 13385 Marseille, France
3
APHM, Service de Radiopharmacie, 13005 Marseille, France
4
Medical Faculty, Aix Marseille University, INSERM, MMG, U 1251, 13385 Marseille, France
5
Medical Faculty, Aix-Marseille University, UR4264, LIIE, 13385 Marseille, France
6
APHM, Service d’Hématologie, Hôpital Conception, 13005 Marseille, France
*
Author to whom correspondence should be addressed.
Academic Editors: Antonio Rodríguez-Sinovas and Gabriel Bidaux
Cells 2021, 10(9), 2305; https://doi.org/10.3390/cells10092305
Received: 28 July 2021 / Revised: 31 August 2021 / Accepted: 31 August 2021 / Published: 3 September 2021
Ischemic vascular diseases are associated with elevated tissue expression of angiomotin (AMOT), a promising molecular target for PET imaging. On that basis, we developed an AMOT-targeting radiotracer, 68Ga-sCD146 and performed the first in vivo evaluation on a myocardial infarction mice model and then, compared AMOT expression and αvβ3-integrin expression with 68Ga-sCD146 and 68Ga-RGD2 imaging. After myocardial infarction (MI) induced by permanent ligation of the left anterior descending coronary artery, myocardial perfusion was evaluated by Doppler ultrasound and by 18F-FDG PET imaging. 68Ga-sCD146 and 68Ga-RGD2 PET imaging were performed. In myocardial infarction model, heart-to-muscle ratio of 68Ga-sCD146 imaging showed a significantly higher radiotracer uptake in the infarcted area of MI animals than in sham (* p = 0.04). Interestingly, we also observed significant correlations between 68Ga-sCD146 imaging and delayed residual perfusion assessed by 18F-FDG (* p = 0.04), with lowest tissue fibrosis assessed by histological staining (* p = 0.04) and with functional recovery assessed by ultrasound imaging (** p = 0.01). 68Ga-sCD146 demonstrated an increase in AMOT expression after MI. Altogether, significant correlations of early post-ischemic 68Ga-sCD146 uptake with late heart perfusion, lower tissue fibrosis and better functional recovery, make 68Ga-sCD146 a promising radiotracer for tissue angiogenesis assessment after MI. View Full-Text
Keywords: angiomotin; myocardial infarction; sCD146; gallium; angiogenesis angiomotin; myocardial infarction; sCD146; gallium; angiogenesis
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MDPI and ACS Style

Moyon, A.; Garrigue, P.; Fernandez, S.; Hubert, F.; Balasse, L.; Brige, P.; Hache, G.; Nail, V.; Blot-Chabaud, M.; Dignat-George, F.; Rochais, F.; Guillet, B. Comparison of a New 68Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction. Cells 2021, 10, 2305. https://doi.org/10.3390/cells10092305

AMA Style

Moyon A, Garrigue P, Fernandez S, Hubert F, Balasse L, Brige P, Hache G, Nail V, Blot-Chabaud M, Dignat-George F, Rochais F, Guillet B. Comparison of a New 68Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction. Cells. 2021; 10(9):2305. https://doi.org/10.3390/cells10092305

Chicago/Turabian Style

Moyon, Anaïs, Philippe Garrigue, Samantha Fernandez, Fabien Hubert, Laure Balasse, Pauline Brige, Guillaume Hache, Vincent Nail, Marcel Blot-Chabaud, Françoise Dignat-George, Francesca Rochais, and Benjamin Guillet. 2021. "Comparison of a New 68Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction" Cells 10, no. 9: 2305. https://doi.org/10.3390/cells10092305

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