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Cells
Volume 10
Issue 3
10.3390/cells10030560
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Article

Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres

by
Takanobu Mashiko
1,2,
Koji Kanayama
1,
Natsumi Saito
1,
Takako Shirado
1,
Rintaro Asahi
1,
Masanori Mori
1 and
Kotaro Yoshimura
1,*
1
Department of Plastic Surgery, Jichi Medical University 3311-1, Yakushiji, Shimotsuke-Shi, Tochigi 329-0498, Japan
2
Department of Plastic Surgery, Toranomon Hospital 2-2-2, Toranomon, Minato-Ku, Tokyo 105-8470, Japan
*
Author to whom correspondence should be addressed.
Cells 2021, 10(3), 560; https://doi.org/10.3390/cells10030560
Submission received: 9 January 2021 / Revised: 26 February 2021 / Accepted: 1 March 2021 / Published: 4 March 2021
(This article belongs to the Collection Research on Adipose Stem Cells)
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Abstract

Therapeutic effects of adult stem-cell transplantations are limited by poor cell-retention in target organs, and a reduced potential for optimal cell differentiation compared to embryonic stem cells. However, contemporary studies have indicated heterogeneity within adult stem-cell pools, and a novel culturing technique may address these limitations by selecting those for cell proliferation which are highly functional. Here, we report the preservation of stemness in human adipose-derived stem cells (hASCs) by using microgravity conditions combined with microspheres in a stirred suspension. The cells were bound to microspheres (100−300 μm) and cultured using a wave-stirring shaker. One-week cultures using polystyrene and collagen microspheres increased the proportions of SSEA-3(+) hASCs 4.4- and 4.3-fold (2.7- and 2.9-fold increases in their numbers), respectively, compared to normal culture conditions. These cultured hASCs expressed higher levels of pluripotent markers (OCT4, SOX2, NANOG, MYC, and KLF), and had improved abilities for proliferation, colony formation, network formation, and multiple-mesenchymal differentiation. We believe that this novel culturing method may further enhance regenerative therapies using hASCs.
Keywords: adipose-derived stem cell; microgravity culture; polystyrene microsphere; collagen microsphere; multilineage-differentiating stress-enduring cell adipose-derived stem cell; microgravity culture; polystyrene microsphere; collagen microsphere; multilineage-differentiating stress-enduring cell

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MDPI and ACS Style

Mashiko, T.; Kanayama, K.; Saito, N.; Shirado, T.; Asahi, R.; Mori, M.; Yoshimura, K. Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres. Cells 2021, 10, 560. https://doi.org/10.3390/cells10030560

AMA Style

Mashiko T, Kanayama K, Saito N, Shirado T, Asahi R, Mori M, Yoshimura K. Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres. Cells. 2021; 10(3):560. https://doi.org/10.3390/cells10030560

Chicago/Turabian Style

Mashiko, Takanobu, Koji Kanayama, Natsumi Saito, Takako Shirado, Rintaro Asahi, Masanori Mori, and Kotaro Yoshimura. 2021. "Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres" Cells 10, no. 3: 560. https://doi.org/10.3390/cells10030560

APA Style

Mashiko, T., Kanayama, K., Saito, N., Shirado, T., Asahi, R., Mori, M., & Yoshimura, K. (2021). Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres. Cells, 10(3), 560. https://doi.org/10.3390/cells10030560

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