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Characterization of Early-Onset Finger Osteoarthritis-Like Condition Using Patient-Derived Induced Pluripotent Stem Cells

1
Catholic iPSC Research Center, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
2
Department of Internal Medicine, Division of Rheumatology, Institute of Medical Science, College of Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul 06591, Korea
*
Author to whom correspondence should be addressed.
Academic Editors: Alexander V. Ljubimov and James Adjave
Cells 2021, 10(2), 317; https://doi.org/10.3390/cells10020317
Received: 12 November 2020 / Revised: 10 January 2021 / Accepted: 27 January 2021 / Published: 4 February 2021
(This article belongs to the Special Issue iPS Cells (iPSCs) for Modelling and Treatment of Human Diseases)
Early osteoarthritis (OA)-like symptoms are difficult to study owing to the lack of disease samples and animal models. In this study, we generated induced pluripotent stem cell (iPSC) lines from a patient with a radiographic early-onset finger osteoarthritis (efOA)-like condition in the distal interphalangeal joint and her healthy sibling. We differentiated those cells with similar genetic backgrounds into chondrogenic pellets (CPs) to confirm efOA. CPs generated from efOA-hiPSCs (efOA-CPs) showed lower levels of COL2A1, which is a key marker of hyaline cartilage after complete differentiation, for 21 days. Increase in pellet size and vacuole-like morphologies within the pellets were observed in the efOA-CPs. To analyze the changes occurred during the development of vacuole-like morphology and the increase in pellet size in efOA-CPs, we analyzed the expression of OA-related markers on day 7 of differentiation and showed an increase in the levels of COL1A1, RUNX2, VEGFA, and AQP1 in efOA-CPs. IL-6, MMP1, and MMP10 levels were also increased in the efOA-CPs. Taken together, we present proof-of-concept regarding disease modeling of a unique patient who showed OA-like symptoms. View Full-Text
Keywords: chondrogenesis; human induced pluripotent stem cell; IL-6; MMP1; MMP10 chondrogenesis; human induced pluripotent stem cell; IL-6; MMP1; MMP10
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MDPI and ACS Style

Rim, Y.A.; Nam, Y.; Park, N.; Lee, K.; Jung, H.; Jung, S.M.; Lee, J.; Ju, J.H. Characterization of Early-Onset Finger Osteoarthritis-Like Condition Using Patient-Derived Induced Pluripotent Stem Cells. Cells 2021, 10, 317. https://doi.org/10.3390/cells10020317

AMA Style

Rim YA, Nam Y, Park N, Lee K, Jung H, Jung SM, Lee J, Ju JH. Characterization of Early-Onset Finger Osteoarthritis-Like Condition Using Patient-Derived Induced Pluripotent Stem Cells. Cells. 2021; 10(2):317. https://doi.org/10.3390/cells10020317

Chicago/Turabian Style

Rim, Yeri A., Yoojun Nam, Narae Park, Kijun Lee, Hyerin Jung, Seung M. Jung, Jennifer Lee, and Ji H. Ju 2021. "Characterization of Early-Onset Finger Osteoarthritis-Like Condition Using Patient-Derived Induced Pluripotent Stem Cells" Cells 10, no. 2: 317. https://doi.org/10.3390/cells10020317

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