The Cellular Innate Immune Response of the Invasive Pest Insect Drosophila suzukii against Pseudomonas entomophila Involves the Release of Extracellular Traps

Carrau, Tessa; Thümecke, Susanne; Silva, Liliana M. R.; Perez-Bravo, David; Gärtner, Ulrich; Taubert, Anja; Hermosilla, Carlos; Vilcinskas, Andreas; Lee, Kwang-Zin

doi:10.3390/cells10123320

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The Cellular Innate Immune Response of the Invasive Pest Insect Drosophila suzukii against Pseudomonas entomophila Involves the Release of Extracellular Traps

^1,†

^2,†,

^3,*

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⁵,

³,

^1,2 and

^1,*

Department Pests and Vector Insect Control, Fraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, D-35394 Giessen, Germany

Institute for Insect Biotechnology, Justus Liebig University, Heinrich Buff Ring 26-32, D-35392 Giessen, Germany

Institute of Parasitology, Justus Liebig University, Schubert Strasse 81, D-35392 Giessen, Germany

⁴

Department of Internal Medicine (Pulmonology), University of Giessen and Marburg Lung Center (UGMLC), Member of the German Center for Lung Research (DZL), Aulweg 123, D-35394 Giessen, Germany

⁵

Institute of Anatomy and Cell Biology, Justus Liebig University, Aulweg 123, D-35392 Giessen, Germany

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this study.

Academic Editor: Günther Weindl

Cells 2021, 10(12), 3320; https://doi.org/10.3390/cells10123320

Received: 13 October 2021 / Revised: 17 November 2021 / Accepted: 25 November 2021 / Published: 26 November 2021

(This article belongs to the Special Issue State-of-Art in Innate Immunity)

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Abstract

Drosophila suzukii is a neobiotic invasive pest that causes extensive damage to fruit crops worldwide. The biological control of this species has been unsuccessful thus far, in part because of its robust cellular innate immune system, including the activity of professional phagocytes known as hemocytes and plasmatocytes. The in vitro cultivation of primary hemocytes isolated from D. suzukii third-instar larvae is a valuable tool for the investigation of hemocyte-derived effector mechanisms against pathogens such as wasp parasitoid larvae, bacteria, fungi and viruses. Here, we describe the morphological characteristics of D. suzukii hemocytes and evaluate early innate immune responses, including extracellular traps released against the entomopathogen Pseudomonas entomophila and lipopolysaccharides. We show for the first time that D. suzukii plasmatocytes cast extracellular traps to combat P. entomophila, along with other cell-mediated reactions, such as phagocytosis and the formation of filopodia. View Full-Text

Keywords: cell culture; Drosophila suzukii; hemocytes; plasmatocytes; extracellular traps

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MDPI and ACS Style

Carrau, T.; Thümecke, S.; Silva, L.M.R.; Perez-Bravo, D.; Gärtner, U.; Taubert, A.; Hermosilla, C.; Vilcinskas, A.; Lee, K.-Z. The Cellular Innate Immune Response of the Invasive Pest Insect Drosophila suzukii against Pseudomonas entomophila Involves the Release of Extracellular Traps. Cells 2021, 10, 3320. https://doi.org/10.3390/cells10123320

AMA Style

Carrau T, Thümecke S, Silva LMR, Perez-Bravo D, Gärtner U, Taubert A, Hermosilla C, Vilcinskas A, Lee K-Z. The Cellular Innate Immune Response of the Invasive Pest Insect Drosophila suzukii against Pseudomonas entomophila Involves the Release of Extracellular Traps. Cells. 2021; 10(12):3320. https://doi.org/10.3390/cells10123320

Chicago/Turabian Style

Carrau, Tessa, Susanne Thümecke, Liliana M.R. Silva, David Perez-Bravo, Ulrich Gärtner, Anja Taubert, Carlos Hermosilla, Andreas Vilcinskas, and Kwang-Zin Lee. 2021. "The Cellular Innate Immune Response of the Invasive Pest Insect Drosophila suzukii against Pseudomonas entomophila Involves the Release of Extracellular Traps" Cells 10, no. 12: 3320. https://doi.org/10.3390/cells10123320

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The Cellular Innate Immune Response of the Invasive Pest Insect Drosophila suzukii against Pseudomonas entomophila Involves the Release of Extracellular Traps

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