Effects of Sex and 17 β-Estradiol on Cardiac Fibroblast Morphology and Signaling Activities In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Isolation and Culture
2.2. Collagen Coated Culture Plates
2.3. Estrogen Treatment
2.4. Cyclic Immunofluorescence
2.5. Post-Image Processing
2.6. Statistical Analysis
3. Results
3.1. Sex-Dissagregated Analysis of CF Morphology
3.2. Relative Levels of Fibrotic Related Signaling Proteins
3.3. Nuclear Localization of Mechanosensitive Proteins
3.4. Correlation Analysis of Protein-Protein Interactions
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Watts, K.; Richardson, W.J. Effects of Sex and 17 β-Estradiol on Cardiac Fibroblast Morphology and Signaling Activities In Vitro. Cells 2021, 10, 2564. https://doi.org/10.3390/cells10102564
Watts K, Richardson WJ. Effects of Sex and 17 β-Estradiol on Cardiac Fibroblast Morphology and Signaling Activities In Vitro. Cells. 2021; 10(10):2564. https://doi.org/10.3390/cells10102564
Chicago/Turabian StyleWatts, Kelsey, and William J. Richardson. 2021. "Effects of Sex and 17 β-Estradiol on Cardiac Fibroblast Morphology and Signaling Activities In Vitro" Cells 10, no. 10: 2564. https://doi.org/10.3390/cells10102564