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Open AccessArticle

Evaluation of Production Protocols for the Generation of NY-ESO-1-Specific T Cells

1
Department of Internal Medicine V, Heidelberg University Hospital, 69120 Heidelberg, Germany
2
Department of Hematology, First Affiliated Hospital of Soochow University, Suzhou 215006, China
3
Department of Hematology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550025, China
4
Department of Nuclear Medicine, Heidelberg University Hospital, 69120 Heidelberg, Germany
5
National Center for Tumor Diseases (NCT), German Cancer Consortium (DKTK), 69120 Heidelberg, Germany
6
Department of Immuno-Gene Therapy, Mie University, Tsu 514-8507, Japan
7
Takeda Pharma Vertrieb GmbH & Co. KG, 10117 Berlin, Germany
*
Author to whom correspondence should be addressed.
Cells 2021, 10(1), 152; https://doi.org/10.3390/cells10010152
Received: 9 November 2020 / Revised: 9 January 2021 / Accepted: 11 January 2021 / Published: 14 January 2021
(This article belongs to the Special Issue Current Advances in T-cell-Based Cancer Immunotherapy)
NY-ESO-1-specific T cells have shown promising activity in the treatment of soft tissue sarcoma (STS). However, standardized protocols for their generation are limited. Particularly, cost-effectiveness considerations of cell production protocols are of importance for conducting clinical studies. In this study, two different NY-ESO-1-specific T cell production protocols were compared. Major differences between protocols 1 and 2 include culture medium, interleukin-2 and retronectin concentrations, T cell activation strategy, and the transduction process. NY-ESO-1-specific T cells generated according to the two protocols were investigated for differences in cell viability, transduction efficiency, T cell expansion, immunophenotype as well as functionality. NY-ESO-1-specific T cells showed similar viability and transduction efficiency between both protocols. Protocol 1 generated higher absolute numbers of NY-ESO-1-specific T cells. However, there was no difference in absolute numbers of NY-ESO-1-specific T cell subsets with less-differentiated phenotypes accounting for efficient in vivo expansion and engraftment. Furthermore, cells generated according to protocol 1 displayed higher capacity of TNF-α generation, but lower cytotoxic capacities. Overall, both protocols provided functional NY-ESO-1-specific T cells. However, compared to protocol 1, protocol 2 is advantageous in terms of cost-effectiveness. Cell production protocols should be designed diligently to achieve a cost-effective cellular product for further clinical evaluation. View Full-Text
Keywords: NY-ESO-1-specific T cells; cell production protocols; adoptive cell therapy NY-ESO-1-specific T cells; cell production protocols; adoptive cell therapy
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MDPI and ACS Style

Gong, W.; Wang, L.; Stock, S.; Ni, M.; Schubert, M.-L.; Neuber, B.; Kleist, C.; Hückelhoven-Krauss, A.; Wu, D.; Müller-Tidow, C.; Schmitt, A.; Shiku, H.; Schmitt, M.; Sellner, L. Evaluation of Production Protocols for the Generation of NY-ESO-1-Specific T Cells. Cells 2021, 10, 152. https://doi.org/10.3390/cells10010152

AMA Style

Gong W, Wang L, Stock S, Ni M, Schubert M-L, Neuber B, Kleist C, Hückelhoven-Krauss A, Wu D, Müller-Tidow C, Schmitt A, Shiku H, Schmitt M, Sellner L. Evaluation of Production Protocols for the Generation of NY-ESO-1-Specific T Cells. Cells. 2021; 10(1):152. https://doi.org/10.3390/cells10010152

Chicago/Turabian Style

Gong, Wenjie; Wang, Lei; Stock, Sophia; Ni, Ming; Schubert, Maria-Luisa; Neuber, Brigitte; Kleist, Christian; Hückelhoven-Krauss, Angela; Wu, Depei; Müller-Tidow, Carsten; Schmitt, Anita; Shiku, Hiroshi; Schmitt, Michael; Sellner, Leopold. 2021. "Evaluation of Production Protocols for the Generation of NY-ESO-1-Specific T Cells" Cells 10, no. 1: 152. https://doi.org/10.3390/cells10010152

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