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Article

Interactions between Cellulose and (1,3;1,4)-β-glucans and Arabinoxylans in the Regenerating Wall of Suspension Culture Cells of the Ryegrass Lolium multiflorum

1
ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, University of Melbourne, Parkville, VIC 3010, Australia
2
Sino-Australia Plant Wall Research Centre, State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A&F University, Lin’an 311300, China
3
Biological Optical Microscopy Platform, University of Melbourne, Melbourne, VIC 3010, Australia
4
Peter MacCallum Cancer Centre, Melbourne, VIC 3000, Australia
5
Department of Animal, Plant & Soil Sciences, Latrobe Institute for Agriculture & Food (LIAF), Latrobe University, Melbourne, VIC 3086, Australia
*
Author to whom correspondence should be addressed.
Cells 2021, 10(1), 127; https://doi.org/10.3390/cells10010127
Received: 25 November 2020 / Revised: 4 January 2021 / Accepted: 6 January 2021 / Published: 11 January 2021
(This article belongs to the Special Issue Research on Plant Cell Wall Biology)
Plant cell walls (PCWs) form the outer barrier of cells that give the plant strength and directly interact with the environment and other cells in the plant. PCWs are composed of several polysaccharides, of which cellulose forms the main fibrillar network. Enmeshed between these fibrils of cellulose are non-cellulosic polysaccharides (NCPs), pectins, and proteins. This study investigates the sequence, timing, patterning, and architecture of cell wall polysaccharide regeneration in suspension culture cells (SCC) of the grass species Lolium multiflorum (Lolium). Confocal, superresolution, and electron microscopies were used in combination with cytochemical labeling to investigate polysaccharide deposition in SCC after protoplasting. Cellulose was the first polysaccharide observed, followed shortly thereafter by (1,3;1,4)-β-glucan, which is also known as mixed-linkage glucan (MLG), arabinoxylan (AX), and callose. Cellulose formed fibrils with AX and produced a filamentous-like network, whereas MLG formed punctate patches. Using colocalization analysis, cellulose and AX were shown to interact during early stages of wall generation, but this interaction reduced over time as the wall matured. AX and MLG interactions increased slightly over time, but cellulose and MLG were not seen to interact. Callose initially formed patches that were randomly positioned on the protoplast surface. There was no consistency in size or location over time. The architecture observed via superresolution microscopy showed similarities to the biophysical maps produced using atomic force microscopy and can give insight into the role of polysaccharides in PCWs. View Full-Text
Keywords: Lolium SCC; plant walls; MLG; cellulose; arabinoxylan; superresolution microscopy; electron microscopy Lolium SCC; plant walls; MLG; cellulose; arabinoxylan; superresolution microscopy; electron microscopy
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MDPI and ACS Style

van de Meene, A.v.d.; McAloney, L.; Wilson, S.M.; Zhou, J.; Zeng, W.; McMillan, P.; Bacic, A.; Doblin, M.S. Interactions between Cellulose and (1,3;1,4)-β-glucans and Arabinoxylans in the Regenerating Wall of Suspension Culture Cells of the Ryegrass Lolium multiflorum. Cells 2021, 10, 127. https://doi.org/10.3390/cells10010127

AMA Style

van de Meene Avd, McAloney L, Wilson SM, Zhou J, Zeng W, McMillan P, Bacic A, Doblin MS. Interactions between Cellulose and (1,3;1,4)-β-glucans and Arabinoxylans in the Regenerating Wall of Suspension Culture Cells of the Ryegrass Lolium multiflorum. Cells. 2021; 10(1):127. https://doi.org/10.3390/cells10010127

Chicago/Turabian Style

van de Meene, Allison v.d., Lauren McAloney, Sarah M. Wilson, JiZhi Zhou, Wei Zeng, Paul McMillan, Antony Bacic, and Monika S. Doblin 2021. "Interactions between Cellulose and (1,3;1,4)-β-glucans and Arabinoxylans in the Regenerating Wall of Suspension Culture Cells of the Ryegrass Lolium multiflorum" Cells 10, no. 1: 127. https://doi.org/10.3390/cells10010127

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