Next Article in Journal
Responses of Canopy Growth and Yield of Potato Cultivars to Weather Dynamics in a Complex Topography: Belg Farming Seasons in the Gamo Highlands, Ethiopia
Previous Article in Journal
Influence of Hydroxyapatite Nanoparticles on Germination and Plant Metabolism of Tomato (Solanum lycopersicum L.): Preliminary Evidence
Previous Article in Special Issue
Mycorrhizal Fungi Enhance Resistance to Herbivores in Tomato Plants with Reduced Jasmonic Acid Production
Article Menu

Export Article

Open AccessArticle
Agronomy 2019, 9(4), 162; https://doi.org/10.3390/agronomy9040162

Biofertilizer Production for Agronomic Application and Evaluation of Its Symbiotic Effectiveness in Soybeans

1
Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
2
Department of Agronomy, Yezin Agricultural University, Yezin 15013, Myanmar
3
Department of Agriculture, Ministry of Agriculutre, Livestock and Irrigation, Yezin 15011, Myanmar
4
Laboratory of Plant Nutrition, Department of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
*
Author to whom correspondence should be addressed.
Received: 12 February 2019 / Revised: 19 March 2019 / Accepted: 23 March 2019 / Published: 27 March 2019
  |  
PDF [659 KB, uploaded 10 April 2019]
  |  

Abstract

This study was conducted to evaluate the effects of Bradyrhizobium japonicum SAY3-7, Bradyrhizobium elkanii BLY3-8, and Streptomyces griseoflavus P4 on the symbiotic effectiveness of soybeans before biofertilizer production, to produce biofertilizer containing the studied three strains (SAY3-7, BLY3-8, and P4), to test the effectiveness of the biofertilizer on soybean varieties, and to assess the varietal effects and interaction effects between variety and biofertilizer on plant growth, nodulation, nitrogen fixation, nutrient absorption, and seed yield. Nitrogen fixation was measured using the acetylene reduction assay and ureide methods. Contents of nutrients (N, P, K, Ca, and Mg) were also measured to calculate their uptakes. In this study, synergistic effects of nitrogen fixation were induced by combined inoculation with SAY3-7, BLY3-8 and P4 in all tested soybean varieties. Therefore, we assumed that an effective biofertilizer could be produced using these effective bacteria (SAY3-7, BLY3-8, and P4). After making biofertilizer using these effective bacteria, packages were stored at 30 °C. The populations of the bacteria in the biofertilizer were maintained at a density of 1 × 108 colony forming units (cfu) g−1 for P4 and 7 × 109 cells g−1 for Bradyrhizobium. Diluting biofertilizer by 10−3 proved more effective for nodulation and nitrogen fixation than other dilution treatments. Moreover, this biofertilizer significantly promoted plant growth, nodulation, nitrogen fixation, nutrient uptakes, and seed yield in Yezin-3 and Yezin-6 soybean varieties. Yezin-6 is a more efficient variety than Yezin-3 for improved plant growth, nodulation, nitrogen fixation, nutrient absorption, and seed yield. Taken together, the application of an effective biofertilizer and the use of an efficient soybean variety can play important roles in promoting plant growth, nodulation, nitrogen fixation, and higher seed yield. View Full-Text
Keywords: Bradyrhizobium; Streptomyces griseoflavus; biofertilizer production; biofertilizer application; nodulation; nitrogen fixation; seed yield Bradyrhizobium; Streptomyces griseoflavus; biofertilizer production; biofertilizer application; nodulation; nitrogen fixation; seed yield
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Htwe, A.Z.; Moh, S.M.; Moe, K.; Yamakawa, T. Biofertilizer Production for Agronomic Application and Evaluation of Its Symbiotic Effectiveness in Soybeans. Agronomy 2019, 9, 162.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Agronomy EISSN 2073-4395 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top