Screening, Identification, and Optimization of Fermentation Conditions of an Antagonistic Endophyte to Wheat Head Blight
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Sample Collection and Endophyte Isolation
2.2. Dual Culture Assays for Antagonistic Tests
2.3. Classification of Endophyte Isolates
2.4. Optimization of Fermentation Conditions
2.5. In Vitro Antagonistic Test of Endophyte against F. graminearum
2.6. Statistical Analysis
3. Results
3.1. Identification of Endophytic Bacteria Strain XS-2
3.2. Determination of Antimicrobial Spectrum
3.3. Determination of the Optimal Fermentation Conditions
3.4. Antagonistic in Vitro Test of XS-2 on F. graminearum in Detached Spikelets
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Pathogen | Average Inhibition Zone Diameter (mm) |
---|---|
Colletotrichum gloeosporioides | 22.0 ± 6.8 d |
Rhizoctonia solani | 25.4 ± 6.7 c |
Sclerotium rolfsii | 29.6 ± 1.7 b |
Alternaria alternata | 33.3 ± 0.2 a |
Fusarium moniliforme | 22.3 ± 0.5 c,d |
Colletotrichum truncatum | 23.4 ± 1.1 c,d |
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Zhang, P.; Zhu, Y.; Ma, D.; Xu, W.; Zhou, J.; Yan, H.; Yang, L.; Yin, J. Screening, Identification, and Optimization of Fermentation Conditions of an Antagonistic Endophyte to Wheat Head Blight. Agronomy 2019, 9, 476. https://doi.org/10.3390/agronomy9090476
Zhang P, Zhu Y, Ma D, Xu W, Zhou J, Yan H, Yang L, Yin J. Screening, Identification, and Optimization of Fermentation Conditions of an Antagonistic Endophyte to Wheat Head Blight. Agronomy. 2019; 9(9):476. https://doi.org/10.3390/agronomy9090476
Chicago/Turabian StyleZhang, Peigen, Yongxing Zhu, Dongfang Ma, Wenjie Xu, Jingjing Zhou, Hanwen Yan, Lei Yang, and Junliang Yin. 2019. "Screening, Identification, and Optimization of Fermentation Conditions of an Antagonistic Endophyte to Wheat Head Blight" Agronomy 9, no. 9: 476. https://doi.org/10.3390/agronomy9090476