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Agronomy 2018, 8(10), 209; https://doi.org/10.3390/agronomy8100209

Leaf Eh and pH: A Novel Indicator of Plant Stress. Spatial, Temporal and Genotypic Variability in Rice (Oryza sativa L.)

1
Africa Rice Center, 01 BP 2551, Bouake 01, Ivory Coast
2
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Unité Propre de Recherche Agroécologie et Intensification Durable des cultures Annuelles (UPR AIDA), F-34398 Montpellier, France
3
Agroécologie et Intensification Durable des cultures Annuelles (AIDA), Univ Montpellier, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), 34090 Montpellier, France
4
Centre d’Etude Régional pour l’Amélioration de l’Adaptation à la Sécheresse (CERAAS), BP 3320 Thiès Escale, Senegal
5
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Unité Mixte de Recherche Amélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales (UMR AGAP), 34398 Montpellier, France
6
Amélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales (AGAP), Univ Montpellier, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Institut National de Recherche Agronomique (INRA), Montpellier SupAgro, 34398 Montpellier, France
7
Agricultural Research Institute and Agrotest Fyto, Kroměříž 76701, Czech Republic
8
Institut National de Recherche Agronomique (INRA), Unité Mixte de Recherche (UMR) 1349 Institut de Génétique, Environnement et Protection des Plantes (IGEPP), BP35327, F-35653 Le Rheu CEDEX, France
9
Institut National Polytechnique de Toulouse (INPT), (Institut National de Recherche Agronomique) INRA, University of Toulouse, Unité Mixte de Recherche (UMR) 1248 AGroécologie, Innovations et TeRritoires (AGIR), BP 52627, 31326 Castanet-Tolosan, France
10
School of Materials Science and Engineering, University of New South Wales, Kensington, NSW 2052, Australia
11
Institute of Resource, Ecosystem and Environment of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
12
Discipline of Chemistry, University of Newcastle, Callaghan, NSW 2308, Australia
*
Author to whom correspondence should be addressed.
Received: 7 September 2018 / Revised: 20 September 2018 / Accepted: 26 September 2018 / Published: 29 September 2018
(This article belongs to the Section Farming Sustainability)
Full-Text   |   PDF [3067 KB, uploaded 29 September 2018]   |  

Abstract

A wealth of knowledge has been published in the last decade on redox regulations in plants. However, these works remained largely at cellular and organelle levels. Simple indicators of oxidative stress at the plant level are still missing. We developed a method for direct measurement of leaf Eh and pH, which revealed spatial, temporal, and genotypic variations in rice. Eh (redox potential) and Eh@pH7 (redox potential corrected to pH 7) of the last fully expanded leaf decreased after sunrise. Leaf Eh was high in the youngest leaf and in the oldest leaves, and minimum for the last fully expanded leaf. Leaf pH decreased from youngest to oldest leaves. The same gradients in Eh-pH were measured for various varieties, hydric conditions, and cropping seasons. Rice varieties differed in Eh, pH, and/or Eh@pH7. Leaf Eh increases and leaf pH decreases with plant age. These patterns and dynamics in leaf Eh-pH are in accordance with the pattern and dynamics of disease infections. Leaf Eh-pH can bring new insight on redox processes at plant level and is proposed as a novel indicator of plant stress/health. It could be used by agronomists, breeders, and pathologists to accelerate the development of crop cultivation methods leading to agroecological crop protection. View Full-Text
Keywords: agroecological crop protection; indicator; measurement; method; oxidative stress; plant health; redox potential agroecological crop protection; indicator; measurement; method; oxidative stress; plant health; redox potential
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Husson, O.; Audebert, A.; Benada, J.; Soglonou, B.; Tano, F.; Dieng, I.; Bousset, L.; Sarthou, J.-P.; Joseph, S.; Menozzi, P.; Boulakia, S.; Futakuchi, K. Leaf Eh and pH: A Novel Indicator of Plant Stress. Spatial, Temporal and Genotypic Variability in Rice (Oryza sativa L.). Agronomy 2018, 8, 209.

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