Next Article in Journal
MSA-ResNet: A Neural Network for Fine-Grained Instar Identification of Spodoptera frugiperda Larvae in Smart Agriculture
Previous Article in Journal
Quantitative Source Apportionment and Source-Oriented Health Risk of Heavy Metals in Soils: A Case Study of Yutian County in the Southern Margin of Tarim Basin, China
Previous Article in Special Issue
Free- and Bound-Form Terpenes in Sweet Potato Peel and Their Antifungal Activity Against Aspergillus flavus-Induced Tomato Spoilage
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Agronomy
Volume 15
Issue 12
10.3390/agronomy15122722
agronomy-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Genome-Wide Identification, Characterization, and Expression Profiles of the CCO Gene Family in Raphanus sativus L. Response to Fusarium oxysporum Resistance

by
Yinbo Ma
1,†,
Wei Zuo
1,†,
Piaopiao Shi
1,
Xinyi Cao
1,
Yuchen Xia
1,
Run Tan
1,
Shuai Hao
1,
Hanyan Sun
1 and
Wenxing Hu
2,*
1
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China
2
College of Plant Protection, Yangzhou University, Yangzhou 225009, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Agronomy 2025, 15(12), 2722; https://doi.org/10.3390/agronomy15122722
Submission received: 16 October 2025 / Revised: 19 November 2025 / Accepted: 24 November 2025 / Published: 26 November 2025
(This article belongs to the Special Issue Phytopathogens and Crop Diseases)
Versions Notes

Abstract

Carotenoid cleavage oxygenases (CCOs) constitute a pivotal enzyme family that catalyze the oxidative cleavage of carotenoids to produce apocarotenoids, which are important signaling molecules involved in plant development, stress adaptation, and immunity responses. Notably, several CCOs, particularly NCED members, contribute to pathogen resistance by mediating abscisic acid (ABA) biosynthesis and apocarotenoid-derived defense signaling. While this gene family has been characterized in diverse plant species, its composition and functions in radish (Raphanus sativus L.) remain poorly understood. This study presents the comprehensive, genome-wide identification of the CCO gene family in radish, revealing 19 putative RsCCO members. A combination of phylogenetic, gene structure, chromosomal localization, and synteny analyses revealed conserved evolutionary patterns and species-specific diversification of the RsCCO family within the Brassicaceae. Promoter analysis identified an abundance of cis-acting regulatory elements related to phytohormone signaling, light perception, and stress responses, suggesting complex transcriptional regulation. Crucially, transcriptomic profiling following infection with Fusarium oxysporum revealed that two genes, RsNCED6 and RsNCED9b, were significantly upregulated in a resistant radish inbred line (‘T5’) compared to a susceptible line (‘T24’), identifying them as key candidate genes involved in ABA-mediated defense responses. This work provides a foundational characterization of the radish CCO gene family, offering novel insights into its role in carotenoid metabolism and pathogen defense.
Keywords: CCO gene family; radish; fusarium wilt CCO gene family; radish; fusarium wilt

Share and Cite

MDPI and ACS Style

Ma, Y.; Zuo, W.; Shi, P.; Cao, X.; Xia, Y.; Tan, R.; Hao, S.; Sun, H.; Hu, W. Genome-Wide Identification, Characterization, and Expression Profiles of the CCO Gene Family in Raphanus sativus L. Response to Fusarium oxysporum Resistance. Agronomy 2025, 15, 2722. https://doi.org/10.3390/agronomy15122722

AMA Style

Ma Y, Zuo W, Shi P, Cao X, Xia Y, Tan R, Hao S, Sun H, Hu W. Genome-Wide Identification, Characterization, and Expression Profiles of the CCO Gene Family in Raphanus sativus L. Response to Fusarium oxysporum Resistance. Agronomy. 2025; 15(12):2722. https://doi.org/10.3390/agronomy15122722

Chicago/Turabian Style

Ma, Yinbo, Wei Zuo, Piaopiao Shi, Xinyi Cao, Yuchen Xia, Run Tan, Shuai Hao, Hanyan Sun, and Wenxing Hu. 2025. "Genome-Wide Identification, Characterization, and Expression Profiles of the CCO Gene Family in Raphanus sativus L. Response to Fusarium oxysporum Resistance" Agronomy 15, no. 12: 2722. https://doi.org/10.3390/agronomy15122722

APA Style

Ma, Y., Zuo, W., Shi, P., Cao, X., Xia, Y., Tan, R., Hao, S., Sun, H., & Hu, W. (2025). Genome-Wide Identification, Characterization, and Expression Profiles of the CCO Gene Family in Raphanus sativus L. Response to Fusarium oxysporum Resistance. Agronomy, 15(12), 2722. https://doi.org/10.3390/agronomy15122722

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop