Lack of Significant Effects of Glyphosate on Glyphosate-Resistant Maize in Different Field Locations
, José Cristimiano dos Santos Neto 2, Cleber Daniel de Goes Maciel 2, Leandro Tropaldi 3, Caio Antonio Carbonari 4, Stephen Oscar Duke 5,* and Leonardo Bianco de Carvalho 1Round 1
Reviewer 1 Report
In this paper, Vitor Bidóia et al., investigated if the application of recommended rates of glyphosate (single and sequential) were used on Glyphosate- Resistant maize have significant effects on growth, foliar mineral content, crop yield, grain quality, in the level of residues in grain of glyphosate and aminomethylphosphonic acid (AMPA).
The research is well designed, the applied methodology is adequate, the results are presented clearly, and the discussion is adequate.
Indeed, the research answers to all five questions.
Discussion : lines 332-334.suggestion: join EFSA (European Food Safety Agency) the most strict food agency in the world
In Science, the item Conclusion is too ambitious (first paragraph - we don't know what will be published today, this week, or next week,....). I do recommend the first paragraph be rewritten.
Suggestion: Although it is not the culture object of the study, since soybean is used in the discussion, place it in the conclusions as well.
Only minor technical errors were observed
Pag.
70 – 42%-clay – 42% clay,
71 – sande – sand
100 – tem – stem
133 – 5 – g – 5 g
161 – diameterwith – diameter with
174 – kg-1- - kg-1
189 - (Figure 3). ). - (Figure 3).
194 – yeild – yield
214 – application (Control) – Type of letter
299 – 16,46,47 - 16, 46, 47
310 – Italian ryegrass is Lolium multiflorum L.
References
Join DOI to the references, when they exist.
38. Jalal, A., …………………2021,207,111225. https://doi.org/10.1016/j.ecoenv.2020.111225.
Comments for author File: 
Comments.pdf
Author Response
RESPONSES TO REVIEWERS IN BOLD CAPITALS
Reviewer 1
Discussion : lines 332-334.suggestion: join EFSA (European Food Safety Agency) the most strict food agency in the world ADDED
In Science, the item Conclusion is too ambitious (first paragraph - we don't know what will be published today, this week, or next week,....). I do recommend the first paragraph be rewritten. WE DISAGREE. THE EVIDENCE IN THIS PAPER AND OTHERS, PLUS THE HIGH YIELD OF GR MAIZE, IS STRONG EVIDENCE FOR A CONCLUSION. WE ADDED A FEW WORDS TO THE CONCLUSIONS.
Suggestion: Although it is not the culture object of the study, since soybean is used in the discussion, place it in the conclusions as well. WE HAVE DONE THIS
Only minor technical errors were observed
Pag.
70 – 42%-clay – 42% clay, CHANGE MADE
71 – sande – sand CHANGE MADE
100 – tem – stem CHANGE MADE
133 – 5 – g – 5 g CHANGE NOT MADE – AS WRITTEN, IT DIFFERENTIATES BETWEEN FIVE SAMPLES OF ONE GRAM AND SAMPLES OF 5 GRAMS
161 – diameterwith – diameter with CHANGE MADE
174 – kg-1- - kg-1 CHANGE MADE
189 - (Figure 3). ). - (Figure 3). CHANGE MADE
194 – yeild – yield CHANGE MADE
214 – application (Control) – Type of letter CHANGE MADE
299 – 16,46,47 - 16, 46, 47 CHANGE MADE
310 – Italian ryegrass is Lolium multiflorum L. CHANGE MADE
References
Join DOI to the references, when they exist. DONE
- 38. Jalal, A., …………………2021,207,111225. https://doi.org/10.1016/j.ecoenv.2020.111225.
Author Response File: Author Response.pdf
Reviewer 2 Report
Article untitled „Lack of Significant Effects of Glyphosate on Glyphosate-Resistant Maize in Different Field Locations” is a very broad and threaded manuscript, however this is another article written by the authors on a very similar topic. The manuscript presents very well and accurately results based on the effect of glyphosate on the composition of glyphosate-resistant corn samples and the quality and yield of corn crops. List of references used is impressive. Abstract, methodology, statistical processing, presentation of the results are impeccable. The article is a laudatory sonata on glyphosate. Please explain in the Introduction and Discussion why so much research on glyphosate is being conducted. Currently, it is an indispensable element of almost each crop in the world. If the glyphosate (and AMPA) are almost not detected in plant material what happen with glyphosate? You have written that “glyphosate translocates well to all metabolic sinks”. What is “the other side of the coin” of its use? Why the allowable concentration of glyphosate is established by International Standard of Food Agriculture Organization of the United Nations and the World Health Organization on 5 μg g−1? The article has been presented very carefully and logically, however the re is a gap - where and how the so-called control samples were collected? Whether traditional fertilizers were used in cultivation “glyphosated crops” and control crops, what was their composition? Were the control samples taken from the same genetic type of maize. How far was the control field from the glyphosate-treated fields?
Author Response
Reviewer 2
responses are capitalized in bold
Article untitled „Lack of Significant Effects of Glyphosate on Glyphosate-Resistant Maize in Different Field Locations” is a very broad and threaded manuscript, however this is another article written by the authors on a very similar topic. The manuscript presents very well and accurately results based on the effect of glyphosate on the composition of glyphosate-resistant corn samples and the quality and yield of corn crops. List of references used is impressive. Abstract, methodology, statistical processing, presentation of the results are impeccable. The article is a laudatory sonata on glyphosate.
Please explain in the Introduction and Discussion why so much research on glyphosate is being conducted. Currently, it is an indispensable element of almost each crop in the world. DONE
If the glyphosate (and AMPA) are almost not detected in plant material what happen with glyphosate? GOOD QUESTION. WE HAVE ADDED SOME DISSUSION ON THIS.
You have written that “glyphosate translocates well to all metabolic sinks”. What is “the other side of the coin” of its use? WE ARE NOT SURE WHAT THE REVIEWER IS ASKING. THE FACT THAT GLYPHOSATE TRANSLOCATES WELL TO ALL METABOLIC SINKS HAS BEEN WELL ESTABLISHED FOR DECADES. WE HAVE ADDED A STATEMENT ABOUT WHY THIS CONTRIBUTES TO THE EFFICACY OF GLYPHOSATE. WE HAVE ADDED QUITE A BIT MORE ABOUT TRANLOCATION OF GLPHOSATE IN MAIZE WHERE THE GLYPHOSAE TRANSLOCATES TO.
Why the allowable concentration of glyphosate is established by International Standard of Food Agriculture Organization of the United Nations and the World Health Organization on 5 μg g−1? WE ARE NOT REGULATORY SCIENTISTS AND DO NOT KNOW THE ANSWER TO THIS QUESTION. THIS NUMBER IS ALMOST CERTAINLY BASED ON A COMBINATION OF ACUTE AND CHRONIC TOXICITY STUDIES, AS WELL AS EXPECTED HUMAN CONSUMPTION OF MAIZE. WE ADDED THE EFSA MAXIMUM RESIDUE LEVEL TO THE PAPER, WHICH IS LOWER.
The article has been presented very carefully and logically, however there is a gap - where and how the so-called control samples were collected? THE CONTROL PLANTS WERE GROWN IN THE SAME LOCATIONS AS THE TREATED PLANTS. THEY WERE NOT SPRAYED WITH GLYPHOSATE. THIS IS MADE CLEARER IN THE M&M NOW.
Whether traditional fertilizers were used in cultivation “glyphosated crops” and control crops, what was their composition? WE USED DIFFERENT FERTILIZERS IN EACH LOCATION AND YEAR, AIMING TO PROVIDE A HIGH-PRODUCTIVITY. FOR EXAMPLE, IN JABOTICABAL, WE USED 8-32-16 (NPK) THE FIRST YEAR AND 4-14-8 (NPK) THE SECOND YEAR. SINCE THIS IS IRRELEVANT TO THE STUDY, WE HAVE LEFT THIS INFORMATON OUT.
Were the control samples taken from the same genetic type of maize. YES, THE CONTROLS WERE THE EXACT SAME GR VARIETY AS THE TREATED. THE OBJECTIVE WAS NOT TO COMPARE VARIETIES BUT TO DETERMINE THE EFFECTS OF GLYPHSOATE ON A SINGLE VARIETY.
How far was the control field from the glyphosate-treated fields? THE CONTROL TREATMENTS WERE RANDOMIZED WITH THE GLYPHOSATE TREATMENTS WITHIN BLOCKS, IN EACH LOCATION. FOR GLYPHOSATE APPLICATION, WE PROTECTED THE SIDES OF THE PLOTS USING PLASTIC TARPS TO PREVENT HERBICIDE DRIFT TO OTHER PLOTS. THE CONTROL SAMPLES WERE COLLECTED FROM THE TWO CENTRAL LINES OF THE CONTROL PLOTS. THIS INFORMATION WAS ADDED TO THE TEXT.
Reviewer 3 Report
Well prepared paper. It looks for the future problem and discussion about glyphosate use.
My suggestions are:
Line 100: plant stem
Line 118: obtained
Line 124: the weight of one thousand grains (not hundred) because the weight is more than 300 grams
Line 134: method, (no dot)
Line 161: diameter with
Line 187: thousand grains
Line 191: thousand not hundred grams but grains
Figure 3B: Weight of one thousand grains
Line 197: thousand grains (B)
Line 214: application (Control) - different fonts
Line 250: of 1000 grains.
Line 368: use soybeans (without dot)
Line 395: Filho, R.V., (without comma)
Line 417: Brassica
Line 434: change = to -
Line 472: Environ. Sci.
Author Response
Reviewer 3
responses are capitalized in bold
Well prepared paper. It looks for the future problem and discussion about glyphosate use.
My suggestions are:
Line 100: plant stem CHANGE MADE
Line 118: obtained CHANGE MADE
Line 124: the weight of one thousand grains (not hundred) because the weight is more than 300 grams WE ARE GLAD YOU FOUND THIS ERROR – CHANGE MADE
Line 134: method, (no dot) CHANGE MADE
Line 161: diameter with CHANGE MADE
Line 187: thousand grains CHANGE MADE
Line 191: thousand not hundred grams but grains CHANGE MADE
Figure 3B: Weight of one thousand grains CHANGE MADE
Line 197: thousand grains (B) CHANGE MADE
Line 214: application (Control) - different fonts CHANGE MADE
Line 250: of 1000 grains. CHANGE MADE
Line 368: use soybeans (without dot) CHANGE MADE
Line 395: Filho, R.V., (without comma) CHANGE MADE
Line 417: Brassica CHANGE MADE
Line 434: change = to - CHANGE MADE
Line 472: Environ. Sci. CHANGE MADE
