Marker-Assisted Backcross Breeding for Improving Bruchid (Callosobruchus spp.) Resistance in Mung Bean (Vigna radiata L.)
, Qinxue Zhang 3, Yun Lin 1,2, Jingbin Chen 1,2, Prakit Somta 4, Qiang Yan 1,2, Chenchen Xue 1,2, Jinyang Liu 1,2, Xin Chen 1,2 and Xingxing Yuan 1,2,*Round 1
Reviewer 1 Report
The manuscript detailed the development of the mungbean line's resistance to bruchid through MABC. The work is easy to follow. This is an important trait to improve in mungbean. However, some areas need to be improved before a recommendation to publish.
Introduction
- The introduction part has failed to impress upon the current gaps in the need for MAS-Mungbean-Bruchids. Add more details.
Materials and Methods
- In section 2.2. Marker-assisted backcross (MABC) breeding strategy for bruchid resistance; Add details about season and year for population development.
- Add the product size for foreground markers VrBR-SSR013 and DMB-SSR158 and prepare the separate supplementary table.
- In section 2.6. Investigation of agronomic trait; Add the reference for traits descriptors
Results
- Section 3.1. The development of bruchid-resistant lines through MABC breeding required substantial improvement. Add the F1, BC1 F1, BC2 F1, BC3 F1, BC3F2, BC3F6 population details such as the total number of plants, how many foreground positive plants, and what basis foreground positive plants were forwarded to the next generation.
- Authors performed background analysis in all the generations but no details about recovery percentage. Add separate main table about recovery percentage in BC1 F1, BC2 F1, BC3 F1, BC3F2, BC3F6 population
- Add the gel images for several foreground positives in F1, BC2 F1, BC3 F1, BC3F2 along with parents. Also, add the graphical genotypes image for improved resistance lines using background data.
- Figures 3 and 4: It would be better to include the image of donor parent V2802
- Table 2: Include the standard error deviation and CD value in the table
- Add morphological evaluation images for those discussed traits.
Discussion
- Line 240-269, too much general information. I suggest reducing and moving a few informations to the Introduction section. The results are to be examined and interpreted more critically. I agree that MABC in mungbean is limited, in this case, authors should discuss their results with other crops
Author Response
Please see the attachment.
Author Response File: 
Author Response.docx
Reviewer 2 Report
The manuscript "Marker-assisted Backcross Breeding for Improving Bruchid (Callosobruchus spp.) Resistance in Mung Bean (Vigna radiata L.)" described the breeding of new mung bean lines with high Bruchid resistance and yield comparable with the recurrent parent.
The manuscript contained sufficient details about the breeding of the resistant lines.
Although it is better to sequence all 3 resistance lines to get a better conclusion about the MAS, it is not necessary based on the current context of the manuscript.
An estimation about the homozygosity at the whole genome level would be a good addition to support the stability of the genome for application.
The manuscript is needed to be copy-edited by Professional or native speak to correct some writing issues.
Author Response
Please see the attachment.
Author Response File: Author Response.docx
