Genome-Wide Association Mapping for Stomata and Yield Indices in Bread Wheat under Water Limited Conditions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Phenotyping
2.3. Statistical Analysis
2.4. Genotyping of Bread Wheat Genotypes
2.5. Population Structure
2.6. GWAS Analysis
3. Results
3.1. Phenotypic Evaluation
3.2. Population Structure
3.3. Genome-Wide Marker-Trait Associations (MTA)
3.4. Stomata Indices
3.5. Yield-Related Indices
3.6. Genome-Wide Multiple Traits Loci Associations
4. Discussions
4.1. Phenotypic Evaluation
4.2. Population Structure
4.3. Genome-Wide Marker-Traits Associations
4.4. Genome-Wide Multiple Traits Loci Associations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Source | DF/ Season | FLA | SS | SF | LV | NGS | GYP | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
S1 | S2 | S1 | S2 | S1 | S2 | S1 | S2 | S1 | S2 | S1 | S2 | ||
REP | 2 | 958 | 805 ** | 6.53 | 7.41 | 4.04 | 2.77 | 5.65 | 6.48 | 43.36 | 42.18 | 40.48 | 62.85 |
GET | 95 | 46.8 ** | 56.0 ** | 36.2 ** | 34.6 ** | 36.66 ** | 36.05 ** | 36.30 ** | 35.46 ** | 297.18 ** | 280.91 ** | 147.58 ** | 143.89 ** |
LEL | 1 | 9331 ** | 9121 ** | 4090.8 * | 4196.9 | 3457.44 * | 3559.24 * | 581.77 * | 490.11 ** | 570.25 * | 415.06 | 441 ** | 424.61 * |
GET × LEL | 95 | 6.44 ** | 8.22 ** | 18.43 ** | 18.09 ** | 16.38 ** | 20.29 ** | 17.95 ** | 19.28 ** | 137.13 ** | 149 ** | 100.08 ** | 106.65 ** |
Error | 382 | 2.91 | 5.01 | 4.99 | 5.19 | 5.4 | 4.32 | 5.06 | 5.2 | 48.27 | 49.66 | 28.63 | 27.84 |
Total | 575 | ||||||||||||
Heritability | 73.52 | 81.19 | 69.21 | 65.38 | 54.67 | 61.00 | 68.29 | 65.98 | 64.33 | 60.82 | 57.70 | 56.26 |
Table | FLA | SS | SF | LV | NGS | GYP | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
N | WS | N | WS | N | WS | N | WS | N | WS | N | WS | |
Mean | 33.2 | 18.2 | 3699 | 3480 | 13.25 | 12.7 | 7.81 | 7.54 | 51.5 | 33 | 22.50 | 15.4 |
Variance | 4.13 | 5.91 | 10.69 | 13.16 | 10.69 | 10.71 | 16.76 | 10.96 | 89.97 | 99.9 | 49.39 | 52.31 |
SE | 0.21 | 0.25 | 0.33 | 0.37 | 0.33 | 0.36 | 0.41 | 0.32 | 0.96 | 0.99 | 0.71 | 0.74 |
Traits | Env. | FLA | SS | SF | LV | NGS |
---|---|---|---|---|---|---|
SS | N | −0.22 | ||||
WS | −0.20 | |||||
SF | N | 0.36 * | 0.39 * | |||
WS | 0.38 * | 0.41 * | ||||
LV | N | 0.45 * | 0.34 | 0.91 ** | ||
WS | 0.37 * | 0.33 | 0.84 ** | |||
NGS | N | 0.76 ** | −0.22 | 0.56 ** | 0.56 ** | |
WS | 0.77 ** | 0.18 | 0.91 ** | 0.89 ** | ||
GYP | N | 0.88 ** | −0.09 | 0.88 ** | 0.92 ** | 0.56 ** |
WS | 0.81 ** | −0.12 | 0.84 ** | 0.83 ** | 0.89 ** |
Trait | Normal Conditions | Water Shortage |
---|---|---|
FLA | G-9244 followed by G-9610, G-9883, C-586642 and G-9493 | G-586642 followed by G-9610, G-9493, G-9883and G-9244 |
SS | G-9610 followed by G-9244, G-9883, C-586642 and G-9493 | G-9610 followed by C-586642, G-9883, G-9244 and G-9493 |
SF | G-9610 followed by G-9244, G-9883 C-586642 and G-9493 | G-9610 followed by G-9244, G-9883, C-586642 and G-9493 |
LV | G-9610 followed by C-586642, G-9610, G-9244 and G-9493 | G-9610 followed by G-9244, G-9883, C-586642 and G-9493 |
NGS | G-9244 followed by G-9610, G-9493, C-586642 and G-9883 | G-9610 followed by G-9244, G-9883, C-586642 and G-9493 |
GYP | G-9493 followed by G-9796, G-9610, G-9883 and G-9244 | G-9493 followed by G-9796, G-9610, G-9244 and G-9883 |
Significant MTAs | |||||
---|---|---|---|---|---|
Genome and Chromosome Wise Distribution | Characteristics Wise Distribution | ||||
Genome | Normal | Water Shortage | Traits | Normal | Water Shortage |
A-Genome | Total 75 MTAs (1A = 8, 2A = 14, 3A = 3, 4A = 16, 5A = 9, 6A = 2, 7A = 23) | Total 92 MTAs (1A = 9, 2A = 22, 3A = 3, 4A = 24, 5A = 10, 7A = 24) | FLA | 19 | 50 |
SS | 30 | 29 | |||
B-Genome | Total 86 MTAs (1B = 8, 2B = 4, 3B = 16, 4B = 16, 5B = 11, 6B = 24, 7B = 7) | Total 140 MTAs (1B = 9, 2B = 7, 3B = 14, 4B = 28, 5B = 12, 6B = 57, 7B = 13) | SF | 19 | 41 |
LV | 32 | 37 | |||
D-genome | Total 17 MTAs (1D = 2, 2D = 9, 3D = 3, 6D = 2, 7D = 1) | Total 12 MTAs (2D = 6, 3D = 3, 6D = 3) | NGS | 47 | 50 |
GYP | 31 | 37 |
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Ahmed, H.G.M.-D.; Iqbal, M.N.; Iqbal, M.A.; Zeng, Y.; Ullah, A.; Iqbal, M.; Raza, H.; Yar, M.M.; Sarwar, N.; Imran, M.; et al. Genome-Wide Association Mapping for Stomata and Yield Indices in Bread Wheat under Water Limited Conditions. Agronomy 2021, 11, 1646. https://doi.org/10.3390/agronomy11081646
Ahmed HGM-D, Iqbal MN, Iqbal MA, Zeng Y, Ullah A, Iqbal M, Raza H, Yar MM, Sarwar N, Imran M, et al. Genome-Wide Association Mapping for Stomata and Yield Indices in Bread Wheat under Water Limited Conditions. Agronomy. 2021; 11(8):1646. https://doi.org/10.3390/agronomy11081646
Chicago/Turabian StyleAhmed, Hafiz Ghulam Muhu-Din, Muhammad Nouman Iqbal, Muhammad Arslan Iqbal, Yawen Zeng, Aziz Ullah, Muhammad Iqbal, Humayun Raza, Muhammad Majid Yar, Nadeem Sarwar, Muhammad Imran, and et al. 2021. "Genome-Wide Association Mapping for Stomata and Yield Indices in Bread Wheat under Water Limited Conditions" Agronomy 11, no. 8: 1646. https://doi.org/10.3390/agronomy11081646