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Article

Protective and Curative Effects of Trichoderma asperelloides Ta41 on Tomato Root Rot Caused by Rhizoctonia solani Rs33

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Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt
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Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, Egypt
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Botany and Microbiology Department, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia
*
Author to whom correspondence should be addressed.
Academic Editor: Noam Alkan
Agronomy 2021, 11(6), 1162; https://doi.org/10.3390/agronomy11061162
Received: 26 April 2021 / Revised: 27 May 2021 / Accepted: 4 June 2021 / Published: 6 June 2021
(This article belongs to the Section Pest and Disease Management)
Two molecularly identified tomato isolates, Trichoderma asperelloides Ta41 and Rhizoctonia solani Rs33, were characterized and antagonistically evaluated. The dual culture technique showed that Ta41 had a high antagonistic activity of 83.33%, while a light microscope bioassay demonstrated that the Ta41 isolate over-parasitized the pathogen completely. Under greenhouse conditions, the application of Ta41 was able to promote tomato plant growth and had a significant increase in plant height, root length, and shoot fresh, shoot dry, root fresh, and root dry weight. It also improved chlorophyll content and total phenol content significantly, both in protective and in curative treatments. The protective treatment assay exhibited the lowest disease index (16.00%), while the curative treatment showed a disease index of 33.33%. At 20 days post-inoculation, significant increases in the relative expression levels of four defense-related genes (PR-1, PR-2, PR-3, and CHS) were observed in all Ta41-treated plants when compared with the non-treated plants. Interestingly, the plants treated with Ta41 alone showed the highest expression, with relative transcriptional levels of CHS, PR-3, PR-1, and PR-2 that were, compared with the control, 3.91-, 3.13-, 2.94-, and 2.69-fold higher, respectively, and the protective treatment showed relative transcriptional levels that were 3.50-, 3.63-, 2.39-, and 2.27-fold higher, respectively. Consequently, the ability of Ta41 to promote tomato growth, suppress Rs33 growth, and induce systemic resistance supports the incorporation of Ta41 as a potential bioagent for controlling root rot disease and increasing the productivity of crops, including tomatoes. View Full-Text
Keywords: Trichoderma asperelloides; Rhizoctonia solani; tomato; biological control; defense-related genes Trichoderma asperelloides; Rhizoctonia solani; tomato; biological control; defense-related genes
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MDPI and ACS Style

Heflish, A.A.; Abdelkhalek, A.; Al-Askar, A.A.; Behiry, S.I. Protective and Curative Effects of Trichoderma asperelloides Ta41 on Tomato Root Rot Caused by Rhizoctonia solani Rs33. Agronomy 2021, 11, 1162. https://doi.org/10.3390/agronomy11061162

AMA Style

Heflish AA, Abdelkhalek A, Al-Askar AA, Behiry SI. Protective and Curative Effects of Trichoderma asperelloides Ta41 on Tomato Root Rot Caused by Rhizoctonia solani Rs33. Agronomy. 2021; 11(6):1162. https://doi.org/10.3390/agronomy11061162

Chicago/Turabian Style

Heflish, Ahmed A., Ahmed Abdelkhalek, Abdulaziz A. Al-Askar, and Said I. Behiry 2021. "Protective and Curative Effects of Trichoderma asperelloides Ta41 on Tomato Root Rot Caused by Rhizoctonia solani Rs33" Agronomy 11, no. 6: 1162. https://doi.org/10.3390/agronomy11061162

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