Next Article in Journal
Genotype and Planting Date Influence on Establishment and Growth of Bassia prostrata (L) A.J. Scott in a Semiarid Subtropical Dry Winter Region
Next Article in Special Issue
The Importance of Ion Homeostasis and Nutrient Status in Seed Development and Germination
Previous Article in Journal
Adaptive Response of a Native Mediterranean Grapevine Cultivar Upon Short-Term Exposure to Drought and Heat Stress in the Context of Climate Change
Previous Article in Special Issue
Trichoderma parareesei Favors the Tolerance of Rapeseed (Brassica napus L.) to Salinity and Drought Due to a Chorismate Mutase
Article

RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops

1
Departament de Biologia Vegetal, Facultat de Farmàcia, Universitat de València, 46100 Burjassot (Valencia), Spain
2
Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universitat de València. 46100 Burjassot (Valencia), Spain
3
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de València, 46100 Burjassot (Valencia), Spain
4
Instituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, 46022 Valencia, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Agronomy 2020, 10(2), 250; https://doi.org/10.3390/agronomy10020250
Received: 19 December 2019 / Revised: 21 January 2020 / Accepted: 5 February 2020 / Published: 8 February 2020
Salt stress drastically reduce crop productivity. In order to identify genes that could improve crop salt tolerance, we randomly expressed a cDNA library of the halotolerant sugar beet in a sodium-sensitive yeast strain. We identified six sugar beet genes coding for RNA binding proteins (RBP) able to increase the yeast Na+-tolerance. Two of these genes, named Beta vulgaris Salt Tolerant 3 (BvSATO3) and BvU2AF35b, participate in RNA splicing. The other four BvSATO genes (BvSATO1, BvSATO2, BvSATO4 and BvSATO6) are putatively involved in other processes of RNA metabolism. BvU2AF35b improved the growth of a wild type yeast strain under salt stress, and also in mutant backgrounds with impaired splicing, thus confirming that splicing is a target of salt toxicity. To validate the yeast approach, we characterized BvSATO1 in sugar beet and Arabidopsis. BvSATO1 expression was repressed by salt treatment in sugar beet, suggesting that this gene could be a target of salt toxicity. Expression of BvSATO1 in Arabidopsis increased the plant salt tolerance. Our results suggest that not only RNA splicing, but RNA metabolic processes such as such as RNA stability or nonsense-mediated mRNA decay may also be affected by salt stress and could be biotechnological targets for crop improvement. View Full-Text
Keywords: RNA-binding proteins; salt toxicity; sugar beet; yeast RNA-binding proteins; salt toxicity; sugar beet; yeast
Show Figures

Figure 1

MDPI and ACS Style

Rosa Téllez, S.; Kanhonou, R.; Castellote Bellés, C.; Serrano, R.; Alepuz, P.; Ros, R. RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops. Agronomy 2020, 10, 250. https://doi.org/10.3390/agronomy10020250

AMA Style

Rosa Téllez S, Kanhonou R, Castellote Bellés C, Serrano R, Alepuz P, Ros R. RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops. Agronomy. 2020; 10(2):250. https://doi.org/10.3390/agronomy10020250

Chicago/Turabian Style

Rosa Téllez, Sara, Rodoldphe Kanhonou, Carlos Castellote Bellés, Ramón Serrano, Paula Alepuz, and Roc Ros. 2020. "RNA-Binding Proteins as Targets to Improve Salt Stress Tolerance in Crops" Agronomy 10, no. 2: 250. https://doi.org/10.3390/agronomy10020250

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop