Next Article in Journal
Effect of the Network Structure and Programming Temperature on the Shape-Memory Response of Thiol-Epoxy “Click” Systems
Next Article in Special Issue
The Effect of β-Glycerophosphate Crosslinking on Chitosan Cytotoxicity and Properties of Hydrogels for Vaginal Application
Previous Article in Journal
PEGylated Fluorescent Nanoparticles from One-Pot Atom Transfer Radical Polymerization and “Click Chemistry”
Previous Article in Special Issue
Comparative Efficacies of a 3D-Printed PCL/PLGA/β-TCP Membrane and a Titanium Membrane for Guided Bone Regeneration in Beagle Dogs
Article Menu

Export Article

Open AccessArticle
Polymers 2015, 7(10), 2131-2145;

Maleimide-Functionalized PEI600 Grafted Polyurethane: Synthesis, Nano-Complex Formation with DNA and Thiol-Conjugation of the Complexes for Dual DNA Transfection

Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Chia-Yi 621, Taiwan
Author to whom correspondence should be addressed.
Academic Editor: Jianxun Ding
Received: 2 August 2015 / Revised: 13 October 2015 / Accepted: 15 October 2015 / Published: 23 October 2015
(This article belongs to the Special Issue Functional Polymers for Medical Applications)
Full-Text   |   PDF [3073 KB, uploaded 23 October 2015]   |  


A polyurethane (PU) grafted with small molecular weight polyethylenimine (PEI600) was synthesized. This PU-PEI600 can assemble DNA via electrostatic interactions into nano-sized polymer/DNA complexes. The complexes exhibited great transfection efficiency in delivering DNA along with a reduced cell toxicity comparing to commercial PEI25k (Mw ~25,000). In order to establish a system for concurrently delivering two different DNA or RNA molecules for cell reprogramming (e.g., induced pluripotent stem cells) or the necessity of multi-expression (e.g., double knock down), the PU-PEI600 was further functionalized with maleimide molecules. The novel PU-PEI600-maleimide would still effectively interact with assigned DNA and different functions of PU-PEI600-maleimide/DNA complexes were self-conjugated in presence of a dithiol molecule (1,6-hexanedithiol). In this study, two reporter genes (pEGFP-C2 and pLanRFP-N) were used and evidence of green/red fluorescence co-expression in cells was observed. This article brings a new concept and a practical method for a plurality of different DNA molecules that are more efficient to be concurrently delivered and co-expressed. This method is very helpful in studying cellular multi-regulation or in the treatment of disease with multiple gene defects in vivo. View Full-Text
Keywords: DNA transfection; polyurethane; PEI; maleimide; thiol conjugation DNA transfection; polyurethane; PEI; maleimide; thiol conjugation

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Hung, W.-C.; Cherng, J.-Y. Maleimide-Functionalized PEI600 Grafted Polyurethane: Synthesis, Nano-Complex Formation with DNA and Thiol-Conjugation of the Complexes for Dual DNA Transfection. Polymers 2015, 7, 2131-2145.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top