1. Introduction
Additive manufacturing (AM) is a constantly evolving field [
1,
2]. According to ISO/ASTM 52900:2021 [
3] standard, there are eight categories of AM processes. Commonly used AM technologies are laminated object manufacturing (LOM), material extrusion (MEX), vat photopolymerization (VPP), material jetting (MJT), and powder bed fusion (PBF) [
4,
5,
6,
7]. LOM is a process through which 3D objects are created using paper, plastic, and metals. In the MEX process, the materials are extruded through a nozzle and the objects are built layer by layer. Commercial MEX processes include fused filament fabrication (FFF), which is one of the most popular AM processes [
8,
9], and continuous fibres reinforcement (CFR). In CFR technology, the printer utilizes a second nozzle to lay down a continuous reinforcement of composite fibres within the FFF technology [
10]. In the VPP process, a liquid photopolymer is selectively cured by light-activated polymerization in a vat. Stereolithography (SLA) is one of the most widely used variants of this technology. PBF is a process in which thermal energy selectively fuses regions of the powder bed. One of these technology groups is selective laser sintering (SLS). The MJT process involves droplets of feedstock material being selectively deposited. The PolyJet™ (manufactured by Stratasys, Ltd. (Rehovot, Izrael)) technology is an MJT process. The SLA, SLS, and PolyJet™ technologies are widely used in medical applications [
11,
12,
13]
FFF technology can be used for concept design, modelling, and cost-effective low-volume production [
8,
9,
14,
15]. One possibility of this technology involves the use of 3D printing filaments with conductive additives such as carbon black or carbon nano tubes. To understand the thermoplastic behaviour of these composites, the mechanical and electrical properties of this type of printing materials were investigated.
Electrically conductive polymer composites offer a new alternative to traditional metal-based systems. There is a growing demand for flexible, soft, non-corrosive materials in the emerging fields of robotics, cybernetics, and biomedicine. Metallic fillers are less popular than carbon-based fillers because they often cause clogging problems during FFF printing [
16]. Sensors measuring dynamic mechanical quantities, like force and acceleration, require conductive fibers with relatively high electrical resistance. Interconnections of electrical conductors can also be printed using FFF technology, but unlike sensors, these need to have relatively low specific resistance [
8].
Several thermoplastic conductive composite filaments are already commercially available. The drawback is that the resistance of these filaments is 4–6 orders of magnitude higher than that of pure metal conductors, such as copper or silver [
17]. By analysing the dielectric and thermal properties of twenty-four polymers used in 3D printing (FFF), it was found that, unlike injection moulding, 3D printed objects have structural homogeneity limitations that are strongly dependent on the moisture content of the filaments and the 3D printer setup, which can consequently affect the dielectric properties of the polymers [
18]. The electrical and mechanical properties of conductive composites based on PLA and ABS have been investigated by Pentek et al. and Ujfalusi and co-workers. For use in prosthetics and the development of medical robotic devices, PLA-based and ABS-based conductive materials were found to be suitable [
19,
20]. Carbon nanotubes (CNT)/ABS composite filaments were prepared, and their conductivity was investigated. It was found that the CNT content significantly and non-linearly changed the mechanical and electrical properties of the ABS material [
21].
Conductive polymers with carbon additives are frequently used as strain sensors and other low-voltage sensor types [
22]. In one study, TPU/CNT flexible strain sensors with auxetic structure were invented. The sensors were manufactured via a hybrid method comprising FFF 3D printing and ultrasonic cavitation-enabled treatment [
23]. Three types of periodic configurations for flexible strain sensors were designed. The periodic structures were manufactured from a composite of TPU and carbon black particles [
24]. ABS/carbon black commercially available composite was tested for stress–strain gauge for health monitoring [
25]. Different shapes of strain sensors were invented using neat TPU material and conductive PLA-based composite [
26].
A wide range of medical applications, including electroencephalogram (EEG) sensors, electromyography (EMG) sensors, tactile sensors, and strain sensors, have been successfully developed and manufactured using 3D printing technology. Multi-channel EEG sensors for zebrafish were manufactured and developed using 3D printing by Cho et al. The 3D printed parts were deposited with Ti/Au composite [
27]. In 2019, Velcescu and co-workers presented nine different configurations of flexible 3D printed EEG electrodes. The electrodes were coated with silver/silver chloride [
28]. In 2020, 3D printable dry EEG electrodes with coiled-spring prongs were invented. The printed sensors were produced using an SLA printer [
29]. In a previous study, EMG sensors were used in prostheses, and the signals were transmitted via 3D printed parts [
20,
30]. In 2021, researchers reviewed the use of polymers in sensors. e.g., for medical applications. The mechanical, electrical, and magnetic properties of the 3D printed sensors and specimens were studied. Printing settings have been found to have a major influence on the properties of the finished samples [
31]. Yang et al. invented wearable flexible electronics. Flexible shape-memory devices have been coated with silver nanoparticles or carbon nanotubes [
32]. Three-dimensional printed bionic ears have been invented by Mannor and coworkers. The ears were made from a chondrocyte-seeded alginate hydrogel matrix with electrically conductive silver nanoparticles and an inductive coil antenna and cochlea-shaped electrodes supported on silicone [
33].
This paper investigates the mechanical, electrical, and thermal properties of conductive composite filaments based on PLA, ABS, TPU, and PA. In this study, the tensile, three-point flexural, Charpy impact, and Shore D hardness properties were measured. The resistance, signal transmissibility, and the relationship between resistance and temperature were also investigated. The relationship between cyclic elongation and resistance was examined. Scanning electron microscopy was performed on the Charpy tests’ broken specimens. Cytotoxicity tests were also carried out for each material. These tests are important in determining the suitability of the composite materials under investigation for use as medical sensors, including strain sensors, EEG and EMG sensors, and temperature and tactile sensors for use in prostheses. Strain sensors made of the matrix materials under investigation have been reported in the literature. The majority of these sensors are composites of a flexible substrate layer and a conductive layer in different shapes. The other type of strain sensor described in the literature is made of a single matrix composite with a special structure. Surprisingly, the simple structure tested in this study has not been investigated in previous work, only specialized single matrix composite structures. The aim of the study is to investigate whether the simple structure studied could be used as a sensor. Another aim of this work is the examination of the relationship between layer height and electrical resistivity for the composites tested.
2. Materials and Methods
The tested conductive polymers are based on PLA, ABS, TPU, and PA. The abbreviations of these composites in order are ESD-PLA, ESD-ABS, ESD-TPU, and ESD-Onyx (ESD, electrostatic discharge). For the fabrication of test specimens, FFF technology was used. The mechanical properties (tensile, flexural, Charpy, and Shore-D tests) and electrical properties (resistance, resistance during cyclic tensile tests, resistance during cyclically varying temperature tests, signal transfer capability) of the specimens were investigated. Scanning electron microscopy on Charpy specimens was carried out. The total number of specimens was 142. In
Figure 1 the protocol diagram of the study is illustrated.
2.1. Materials and 3D Printing Parameters
The materials that were the subject of this research comprised the following conductive composites: ESD-PLA (Protopasta CDP11705; Protoplant Inc., 12001 NE 60th Way, Suite B-2,Vancouver, WA 98682, USA), a PLA-based composite with added carbon black; ESD-ABS (ABS Conductive; SUNLU, 92 Corporate Park STE C204 Irvine, 92606, CA, USA), an antistatic ABS filament; ESD-TPU (Conductive Filaflex Black; Recreus Industries, S.L. Polígono, Industrial Finca Lacy, 03600, Elda (Alicante), Spain), a flexible electrically conductive TPU filament filled with carbon black; and an ESD safe PA-based filament with chopped micro carbon fibers, ESD-Onyx (Markforged Onyx ESD; Markforged, 60 Tower Road, Waltham, MA, USA). The diameter of each filament was 1.75 mm.
All samples were produced using FFF technology. For the ESD-PLA, ESD-ABS, and ESD-TPU materials, a Craftbot Plus desktop 3D printer (Craftunique Ltd.; 1143 Budapest, Ilka Street 50, Hungary) was used; for the ESD-Onyx material, a Markforged X7TM industrial CFR printer (Markforged; 60 Tower Road in Waltham, MA, USA) was utilized. All the specimens were printed in XYZ orientation according to ISO/ASTM 52900 standard.
All specimens were printed with 100% fill density, and for the mechanical tests, 200 µm layer height. The other printing parameters and layer heights for electrical tests of the investigated materials are shown in
Table 1.
2.2. Mechanical Analysis
All the mechanical tests were repeated five times for each material, according to the relevant ISO standards. All tests were performed at an ambient temperature of 23 °C ± 1 °C.
2.2.1. Tensile Test
Tensile testing was performed by a Zwick/Roell Z100THW universal test machine (ZwickRoell, 89079, Ulm, Germany). The tests were carried out according to ISO 527-1:2019 standard [
34]. The specimens were 1A from the ISO 527-2:2012. The preload was set to 0.1 MPa; the testing speed was 1 mm/min for the determination of Young’s modulus, and was then set to 50 mm/min for the tests.
2.2.2. Three-Point Flexural Test
The three-point bending test was carried out using the same Zwick/Roell Z100THW universal material tester. The tests were based on the ISO 178:2019 standard [
35] with the preferred test specimen; its size was 80 mm × 10 mm × 4 mm. The preload was 0.1 MPa, and the testing speed was set to 2 mm/min during the full test. The support distance was 64 mm, and the maximal deformation was 4.7%, according to the standard.
2.2.3. Charpy Impact Test
To measure the impact values, the specimens were tested using a Zwick/Roell HIT50P (ZwickRoell, 89079, Ulm, Germany) instrument utilizing a 5 J pendulum, following the ISO 179-1:2010 standard [
36]. The size of the specimen was 80 mm × 10 mm × 4 mm. The edgewise impact was performed on the test specimen without notch.
2.2.4. Shore D Hardness
The Shore D hardness tester was a Zwick/Roell 3131/320154 (Zwick/Roell, 89079, Ulm, Germany). The tests were performed according to ISO 868:2003 standard [
37]. The instrument was set on a stable stand during the entire measurement process.
2.3. Electrical Measurements
2.3.1. Electrical Resistance Measurements
Conductivity measurements were made out using a Voltcraft VC830 digital multimeter (Conrad Electronic SE, Klaus Condrad—Str. D-92240 Hirschau, Germany). Probes were placed at identical locations on the surface of bolt nuts, which were placed on the screws at the end points of the specimens. This method was used for the initial resistance measurements in the cyclic tensile and temperature tests.
2.3.2. Resistance Measurement during Tensile Test
The resistance of the specimens was measured during tensile tests. For the measurement, the same Zwick/Roell Z100THW universal test machine was used, and the resistance was measured throughout the test with an Arduino Uno R1 (Arduino srl., Arduino.cc). The specimens’ dimensions were 50 mm × 5 mm × 0.6 mm, with 3 mm holes at the ends (
Figure 2). First, the specimens were stretched for 10 cycles, and then subjected to a tensile test.
The four materials were tested with four different stretching modes. The strain was set between 0.3 mm and 0.65 mm for ESD-PLA, 0.2 mm and 0.8 mm for ESD-ABS, 0.1 mm and 0.5 mm for ESD-TPU, and 0.7 mm and 1.1 mm for ESD-Onyx. The relationship between the maximum resistance per cycle and the elongation was examined.
The gauge factor of a strain gauge is the ratio of the relative resistance change and relative length change. It is defined as:
where R is the measured resistance during the tensile test [Ω], R
0 is the initial resistance [Ω], and ε is the strain during the tensile test [%].
2.3.3. Resistance–Temperature Measurement
The relationship between the thermal and electrical properties of the composite materials was investigated. The size of the specimens was 50 mm × 5 mm × 0.6 mm. The measurements were repeated for each specimen after 24 h. The measurement was carried out with Arduino Uno R1 as a resistance meter and the heated print bed of a Craftbot Plus desktop 3D printer as a heater. Temperature was measured using a thermistor with 100 kΩ resistance. Kapton tape was used for insulation and fixing of the specimens to the printing bed of the 3D printer. The temperature interval was varied between 10 °C and 50 °C for ESD-PLA, 20 °C and 80 °C for ESD-ABS, 10 °C and 80 °C for ESD-TPU, and 10 °C and 60 °C for ESD-Onyx composites. The sampling frequency was fs = 10 Hz. The measuring equipment (
Figure 3) was identical to the resistance–temperature measurement setup used by Ujfalusi et al. [
20].
2.3.4. Resistance during Flexural Test
The effect of bending the conductive composite on the resistance was investigated. The specimens (mixed flexural specimens) were made from a neat polymer and a composite material. The schematic drawing of the specimen is in
Figure 4. The conductive polymer was surrounded from the bottom and top by the neat polymer, ensuring that the whole of the conductive part remained in the bending zone. The length of the neat polymer parts (35 mm) was shorter than the conductive polymer part (50 mm). The whole specimen had a thickness of 3.6 mm, the conductive part had a thickness of 0.6 mm, and the neat parts had a thickness of 1.8 mm and 0.6 mm. The investigation was implemented using the same Zwick/Roell Z100THW universal material tester for bending the specimens and an Arduino Uno as a resistance meter.
2.3.5. Signal Transfer Capability
To determine the signal transfer, specimens measuring 100 mm × 5 mm × 1.2 mm with 3 mm holes at the ends were prepared (
Figure 5) and a Rohde & Schwarz HMF2525 25 MHz function generator and a Rohde & Schwarz RTB2003 2.5 Gsa/s digital oscilloscope (Rohde & Schwarz USA, Inc. 6821 Benjamin Franklin Drive, Columbia, MD 21046, USA) were used.
Excitation sinus-signals were transferred in the X direction of specimens, with different frequencies that varied between fe = 100 Hz and 500 kHz. The test was performed between 100 Hz and 500 kHz for the ESD-PLA, ESD-TPU, and ESD-Onyx composites, and between 100 Hz and 250 kHz for the ESD-ABS composite. The amplitude was set Ue = 1 V in the cases of the ESD-PLA, ESD-ABS, and ESD-TPU, and Ue = 10 V for ESD-Onyx.
2.4. Scanning Electron Microscopy (SEM)
A JEOL JSM-IT500HR SEM (3–1–2 Musashino, Akishima, Tokyo 196–8558, Japan) was used for SEM tests. For this purpose, the Charpy specimens’ broken surfaces and the intact surfaces of the test bars were scanned with 250×, 1000×, and 15,000× magnification, and Markforged matrix and carbon material with 2000×. Before imaging, the samples were coated with gold using a JEOL JFC-1300 auto-fine coater.
2.5. Cytotoxicity
2.5.1. Cell Culturing and Treatment
A549 cells (ATCC, Manassas, VA, USA, Cat.no: CRM-CCL-185) were cultured in Dulbecco’s modified Eagle’s medium (Lonza, Belgium, Cat. no: 12604F)) at 37 ˚C, in a humidified atmosphere containing 5% CO2. The medium was supplemented with 10% foetal bovine serum (FBS, Euroclone, Italy, Cat. no.: ECS0180L), 100 U/mL of penicillin–streptomycin (Lonza, Belgium, Cat. no.: DE-17-602E), 0.1 mM non-essential amino acids (Lonza, Belgium, Cat. no: 13-114E), 10 mM Hepes (Lonza, Belgium, Cat. no: 17-737E), and diluted ß-mercaptoethanol (Thermo, Waltham, MA, USA, Cat. no: 21985–023). All the cell viability tests were performed in triplicates and repeated in three independent experiments (n = 9). The tested inserts made from various polymer materials were placed into the wells of a 24-well plate. To prevent the contamination of the cell cultures, the surfaces of the tested inserts were UV irradiated for 2 × 30 min on both sides.
A549 cells were resuspended in 1 mL DMEM medium, seeded to the surface of a 24-well plate at 3 × 104 cell/well density, and incubated for 8 h at 37 °C in a humidified atmosphere containing 5% CO2. Once the cells were attached to the surface of the cell culture plate, the printed synthetic inserts were placed into each well and cultured for 48 h. A549 cells treated with 1 µM staurosporine (Sigma Aldrich, Burlington MA, USA, Cat. no: S-6942) were used as positive controls for the detection of apoptotic cell death in the total nucleic acid content determination assay.
2.5.2. Light Microscopy
Light microscopic images were taken by a Nikon Eclipse light microscope using a 20× magnification. Scale bars were layered on the images using ImageJ 1.46 software.
Living cell number quantification was performed based on total nucleic acid content determination.
An applied cell viability assay was conducted based on the detected fluorescence intensity of propidium iodide (PI) (Sigma-Aldrich, Cat. no: 81845) staining. The approach was different from the standard PI staining protocols. After 48 h exposure to the synthetic inserts (ESD-ABS, ESD-PLA, ESD-TPU, and ESD-Onyx), total nucleic acid content was determined by PI staining on the Perkin-Elmer EnSight Multimode plate reader. Briefly, the treated cells were washed in 500 µL of PBS (phosphate buffered saline) (pH 7.4) to remove all the detached apoptotic cells. After removing apoptotic cells, adherent, living cells were lysed in 500 μL cell-lysing borate buffer (pH 9.2) and labelled with PI at 2 μg/mL concentration. After a 5-min incubation on an orbital shaker in a dark environment, fluorescent intensities were measured at λ excitation = 530 nm and λ emission = 620 nm wavelengths. All the treated sample results were compared to the untreated controls.
The analysis of the cytotoxicity results was generated in GraphPad 9 Software. Statistical analysis was performed by one-way ANOVA with the Dunnet post hoc test after data passed the normality test using the Kolmogorov–Smirnov test. Significance levels were labelled as follows: non-significant (ns),
p < 0.0001 (****) [
38].
2.6. Statistical Analysis
The measurement data were assessed and visualized using Origin Pro 2018 (OriginLab Corporation, One Roundhouse Plaza, Suite 303, Northampton, MA 01060, USA). The results of the cyclic tensile tests and resistance–temperature measurements were smoothed using the 15 pts Adjacent Averaging smooth method of the Origin Pro 2018. For the resistance measurement during tensile test, the relationship between the maximum resistance per cycle and the elongation was evaluated using a fitted curve. The fitted function was as follows:
where A
1 is the amplitude (constant) [Ω], t
1 is a constant [mm], and y
0 is the offset [Ω]. In this case, x is the elongation [mm], and y is the fitting curve of the maximum resistances as a function of elongation [Ω]. The limit of the fitting function is y
0 [Ω]. The fit is considered good if the R
2 parameter is above 0.97.
4. Discussion
Conductive polymers are widely used in medical applications and low-voltage solutions. This study investigated the mechanical and electrical properties of conductive polymer samples with carbon-based additives. The composites tested were based on PLA, ABS, TPU, and PA. Mechanical tests included tensile, three-point flexural, Charpy impact and, Shore-D hardness tests. Electrical tests consisted of resistance tests, resistance measurement during cyclic tensile tests, resistance during cyclical variation of temperature tests, resistance during flexural tests, and tests of the signal transmission capability. On the Charpy specimens, scanning electron microscopy was performed. These measurements are necessary to determine whether the composites tested can be used in medical applications such as EEG and EMG sensors and tactile and strain sensors in prosthetics.
Considering the results of the mechanical tests, the most rigid specimens were made of the PA-based, ESD-Onyx composite. The most flexible samples were made of ESD-TPU material.
Based on the electrical measurements, the resistance of the specimens depended on the layer height; if it was greater, the resistance was lower for all tested composites. This relationship was not observed in the case of the ESD-TPU and ESD-Onyx. It was also confirmed by Péntek and coauthors [
19] for similar PLA-based and ABS-based composites with carbon-based additives from another manufacturer; in that paper, only the resistance–temperature relationship was measured.
The change in resistance during the cyclic tensile tests was investigated for all composite materials. The tested composite materials had the property that the electrical resistance changes with the tensile force. The ESD-ABS composite had predictable behaviour in cyclic tensile tests. The relationship between the resistance and the tensile force was clear in a cycle. The maximum of the resistance per cycle exhibited exponential decreasing behaviour. The gauge factor of the specimens from ESD-ABS was relatively high [
39]. This material, with its simple structure, had good strain properties. The examined gauge factor of the ESD-ABS specimens, ranging from 0.35 mm to 0.8 mm elongation, was higher than that found in the literature. The specimens from this composite material with 100 µm layer height can be applied for testing the durability and investigating the failure mechanics and strength of a material in various electronic devices. ESD-ABS could be a future printed material that is applied in various medical devices like flexible pressure sensors, surgical robots, implants, medical imaging, and piezo-resistive sensing devices for prosthetics [
31,
40,
41,
42]. It could also have various extended applications in healthcare industries, as it measures stress–strain tissues during surgery, early detection of stiffness in tissues, stress–strain on prosthetic joints, and early identification of pressure ulcers in patients. Taking into account the above results, fabricating an auxetic structure from this conductive ABS composite, like [
23,
24] suggested, could result in better strain properties than in the results presented.
During cyclic tensile testing, the ESD-TPU samples exhibited specific behaviour. The resistance of the specimens was not monotonic during elongation. After a short increasing phase, the resistance decreased in the elongation phase. In this phase, the ESD-TPU samples had a negative gauge factor, which could be explained by the Poisson factor [
43,
44]. The thickness of the specimen decreased as it stretched, bringing the conductive particles closer together and reducing the resistance. In the cyclic tensile tests, the resistance minima were delayed in relation to the force minima, whereas the maxima coincided for ESD-TPU. This was probably due to the regeneration time of the polymer molecule structure. ESD-PLA and ESD-Onyx composites were also investigated using this test type, but the data showed no valuable results.
The behaviour of the resistance during flexural tests was examined with mixed specimens from neat and conductive polymers for the cases of ESD-ABS and ESD-TPU. Both conductive composites were subjected to a set-up cycle. After this cycle, the resistance was a linear function of the angle between 8°and 18° for ESD-ABS. In the case of ESD-TPU, the resistance decreased with the flexural angle, which could also be due to Poisson’s ratio, as in the case of the resistance–tensile tests.
The relationship between temperature and electrical resistance was investigated for all tested composites. It can be concluded that the ESD-ABS and ESD-TPU materials could be used as raw materials for thermometers. This property has been investigated by Ujfalusi and coworkers for similar ABS-based materials [
20]. For both materials, the electrical resistance was a monotonic function of temperature during heating and cooling. During the tests, the maximum of resistance was decreased from cycle to cycle. The first heating–cooling cycle in the case of the ESD-TPU material was a setup cycle. In the case of the ESD-Onyx, the resistance had a maximum at around 20 °C. The ESD-PLA material was not suitable for this purpose.
The signal transfer capability of the composites was investigated. The results of the experiments showed that ESD-PLA, ESD-ABS, and ESD-TPU can be used as low-pass filters. Péntek and coworkers have found that similar conductive PLA and ABS composite materials could also be used as low-pass filters [
19]. The ESD-Onyx composite is not suitable for electronic signal transmission due to its high attenuation, which was measured in all the layer height cases for this material.
Broken specimens of all materials tested were imaged by scanning electron microscopy. Comparing the results in the literature [
43,
44,
45] and the SEM images of the ESD-ABS’ broken surface, the conductive filler of this material was carbon nanotube. This information was not included in the data published by the manufacturer.
Cell viability tests showed that none of the materials had cytotoxic effects on the cells. A slightly increased cell viability was observed in ESD-ABS, ESD-PLA, and ESD-TPU materials. The highest cell viability was observed in the case of ESD-PLA material, which is a well-known biocompatible and biodegradable plastic material that can potentially support cell viability. The biological properties indicate potential use cases for skin- or short-term contact devices such as sensors or electrodes in biomedical applications. However, before actual clinical use, a detailed characterization is essential in preclinical settings, according to the relevant international standards and ethical considerations.
In summary, composite polymer filaments with carbon-based additives have opened possibilities in low-voltage and medical applications. This study investigates mechanical, electrical, and cell viability properties of commercially available filaments with carbon-based fillers. Based on the results of the resistance measurements during cycle tensile tests and cyclical variation of temperature, as well as signal transmission capability, specimens manufactured from the ABS-based and TPU-based composites are suitable for sensors, such as temperature sensors, strain gauges, and low-voltage medical applications such as EEG and EMG sensors. Based on the results of cytotoxicity tests, low-voltage skin-contact sensors could be made from these materials, facilitating their medical use. The tested conductive materials based on ABS and TPU are appropriate for use as medical sensors, even in the simple form tested.
In terms of limitations, the tested materials were difficult to print. Some specimens were printed more than once due to printing errors. Another limitation of this study was that only one sample structure was examined. Other properties of this block structure (fill density etc.) or other structures should be investigated to improve the applicability of the composites for sensors.
5. Conclusions
Conductive polymers have extensive utilization in the topic of sensors and low-voltage applications. The study investigated the mechanical, electrical, and biological properties of four commercially available conductive polymer composites which incorporate carbon-based additives. The materials of the study were ESD-PLA, ESD-ABS, ESD-TPU, and ESD-Onyx. The tests performed were necessary to verify the suitability of the tested materials for low-voltage applications and use in medical sensors (e.g., temperature sensor, tactile sensor, and strain sensor).
Based on the results of the cyclic tensile tests, the ESD-ABS samples with 100 µm layer height could be used as a stress–strain stamp. These 100 µm layer height specimens have predictable behaviour in a cyclic tensile test. The resistance is unambiguous in the same stress and release branch. The change in resistance in one cycle does not exceed 50% in the 0.2 mm–0.8 mm strain interval, and this change can be called exponential. The gauge factor of these samples is between 40 and 120. Samples of this material with this simple structure have sufficient strain characteristics for use in a strain gauges. Strain sensor fabricated from the ESD-ABS conductive composite can be widely used in medical applications, e.g., prosthetics, ulcer detection, or strain detection in orthosis applications, but when fixing it should be noted that the first cycle is a “set-up cycle”.
According to the results of the resistance during flexural tests, the ESD-ABS composite can be used as a bending sensor if the bending angle is less than 18°.
Based on the cyclic temperature–resistance tests, the ESD-ABS and ESD-TPU composite materials are suitable for raw materials for thermometers and thermosensors, between 20 °C and 75 °C and 10 °C and 80 °C.
Based on the signal transfer capability measurements, the ESD-PLA, ESD-ABS, and ESD-TPU composites can be used as a low-pass filter. These materials can be used as electrocardiography (EEG) and electromyography (EMG) sensors, and in pulse oximeters with a combination of LED and SpO2 sensors to measure blood oxygen saturation. The ESD-Onyx composite is not suitable for use as a wire or other low-voltage electrical component. This material can only be used for ESD safe solutions, such as encapsulation.
According to the results listed, considering that none of the composites investigated are cytotoxic, the ABS-based and TPU-based composites could be suitable for use in a variety of medical sensors. Further studies should investigate strain, bending, and temperature sensors with structures other than those tested in this work using these materials. Strain sensors should be developed with special structures to improve the gauge factor. The further developed sensors could be used in medical sensors in clinical trials, mainly in the field of prosthetics and orthotics. Additionally, they could be considered for future application as an SMP (smart memory polymer) for 4D printing technology in biomedical use cases.