Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration

Sartika, Dewi; Wang, Chih-Hsin; Wang, Ding-Han; Cherng, Juin-Hong; Chang, Shu-Jen; Fan, Gang-Yi; Wang, Yi-Wen; Lee, Chian-Her; Hong, Po-Da; Wang, Chih-Chien

doi:10.3390/polym12040853

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Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration

^1,†,

^2,†,

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⁴,

⁸,

⁹ and

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Laboratory of Adult Stem Cell and Tissue Regeneration, National Defense Medical Center, Taipei 114, Taiwan

Department of Plastic and Reconstructive Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan

Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei 112, Taiwan

⁴

Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, Taipei 114, Taiwan

⁵

Department of Gerontological Health Care, National Taipei University of Nursing and Health Sciences, Taipei 112, Taiwan

⁶

Division of Rheumatology/Immunology/Allergy, Department of Internal Medicine, Tri- Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan

⁷

Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan

⁸

Department of Orthopedics, Taipei Medical University Hospital, Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan

⁹

Department of Materials Sciences and Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan

¹⁰

Department of Orthopedic Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Polymers 2020, 12(4), 853; https://doi.org/10.3390/polym12040853

Received: 28 February 2020 / Revised: 2 April 2020 / Accepted: 5 April 2020 / Published: 7 April 2020

(This article belongs to the Special Issue Polymers for Bone Tissue Engineering)

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Abstract

Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of the SF scaffold with and without seeded human adipose-derived mesenchymal stem cells (hASCs) in facilitating bone regeneration. The effectiveness of the SF-hASCs scaffold was evaluated based on physical characterization, biocompatibility, osteogenic differentiation in vitro, and bone regeneration in critical rat calvarial defects in vivo. The SF scaffold demonstrated superior biocompatibility and significantly promoted osteogenic differentiation of hASCs in vitro. At six and twelve weeks postimplantation, micro-CT showed no statistical difference in new bone formation amongst all groups. However, histological staining results revealed that the SF-hASCs scaffold exhibited a better bone extracellular matrix deposition in the defect regions compared to other groups. Immunohistochemical staining confirmed this result; expression of osteoblast-related genes (BMP-2, COL1a1, and OCN) with the SF-hASCs scaffold treatment was remarkably positive, indicating their ability to achieve effective bone remodeling. Thus, these findings demonstrate that SF can serve as a potential carrier for stem cells, to be used as an osteoconductive bioscaffold for BTE applications. View Full-Text

Keywords: silk fibroin; scaffold; adipose stem cells; bone regeneration; calvarial defects; bone tissue engineering

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MDPI and ACS Style

Sartika, D.; Wang, C.-H.; Wang, D.-H.; Cherng, J.-H.; Chang, S.-J.; Fan, G.-Y.; Wang, Y.-W.; Lee, C.-H.; Hong, P.-D.; Wang, C.-C. Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration. Polymers 2020, 12, 853. https://doi.org/10.3390/polym12040853

AMA Style

Sartika D, Wang C-H, Wang D-H, Cherng J-H, Chang S-J, Fan G-Y, Wang Y-W, Lee C-H, Hong P-D, Wang C-C. Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration. Polymers. 2020; 12(4):853. https://doi.org/10.3390/polym12040853

Chicago/Turabian Style

Sartika, Dewi, Chih-Hsin Wang, Ding-Han Wang, Juin-Hong Cherng, Shu-Jen Chang, Gang-Yi Fan, Yi-Wen Wang, Chian-Her Lee, Po-Da Hong, and Chih-Chien Wang. 2020. "Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration" Polymers 12, no. 4: 853. https://doi.org/10.3390/polym12040853

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Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration

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