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Open AccessArticle

Neutral Polysaccharide from the Leaves of Pseuderanthemum carruthersii: Presence of 3-O-Methyl Galactose and Anti-Inflammatory Activity in LPS-Stimulated RAW 264.7 Cells

1
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam
2
Department of Pharmacy, Section of Pharmaceutical Chemistry, University of Oslo, 0316 Oslo, Norway
3
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam
4
Friedrich Schiller University of Jena, Institute for Organic Chemistry and Macromolecular Chemistry, Center of Excellence for Polysaccharide Research, Humboldtstrasse, D-07743 Jena, Germany
5
Key Laboratory for Enzyme and Protein Technology, Hanoi University of Science, Hanoi, Vietnam
*
Author to whom correspondence should be addressed.
Polymers 2019, 11(7), 1219; https://doi.org/10.3390/polym11071219
Received: 18 June 2019 / Revised: 15 July 2019 / Accepted: 16 July 2019 / Published: 22 July 2019
(This article belongs to the Collection Polysaccharides)
Pseuderanthemum carruthersii (Seem.) Guillaumin is a native tree in Vietnam. The water extract of the leaves from this tree gives a highly viscous product that has been used to heal wounds and treat inflammations. Our previous studies showed that the leaves of P. carruthersii have a high content of polysaccharides. In this study, the structure and influence of the neutral polysaccharide from Pseuderanthemum carruthersii (PCA1) on lipopolysaccharide (LPS)-stimulated RAW264.7 cells were investigated. The PCA1 isolated from P. carruthersii is a galactan-type polysaccharide, containing galactose (77.0%), 3-O-methyl galactose (20.0%), and arabinose (3.0%). Linkage analysis of PCA1 showed that both the 3-O-methyl galactose and galactose were 1,4-linked. The presence of 3-O-methyl galactose units as part of the polysaccharide is important and can be used as a chemotaxonomic marker. The molecular weight of the PCA1 was 170 kDa. A PCA1 concentration of 30–40 μg/mL strongly inhibited TNFα, IL-1β, and IL-6 inflammatory cytokine production, and reactive oxygen species (ROS) release. PCA1 had inhibitory activities on pro-inflammatory cytokine and ROS release in LPS-stimulated mouse macrophages in vitro through MAPK signaling. View Full-Text
Keywords: Pseuderanthemum carruthersii; 3-O-methyl galactose; inflammation; MAPK; polysaccharides Pseuderanthemum carruthersii; 3-O-methyl galactose; inflammation; MAPK; polysaccharides
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Bac, V.H.; Paulsen, B.S.; Truong, L.V.; Koschella, A.; Trinh, T.C.; Wold, C.W.; Yogarajah, S.; Heinze, T. Neutral Polysaccharide from the Leaves of Pseuderanthemum carruthersii: Presence of 3-O-Methyl Galactose and Anti-Inflammatory Activity in LPS-Stimulated RAW 264.7 Cells. Polymers 2019, 11, 1219.

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