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Article

Synthesis and Characterization of Isosorbide-Based Polyurethanes Exhibiting Low Cytotoxicity Towards HaCaT Human Skin Cells

1
CERENA, Departamento de Engenharia Química, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
2
Research Institute for Medicine and Pharmaceutical Science (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal
3
Centro de Química Estrutural, Departamento de Engenharia Química, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
*
Authors to whom correspondence should be addressed.
Polymers 2018, 10(10), 1170; https://doi.org/10.3390/polym10101170
Received: 21 September 2018 / Revised: 12 October 2018 / Accepted: 17 October 2018 / Published: 20 October 2018
(This article belongs to the Special Issue Functional Polyurethanes – In Memory of Prof. József Karger-Kocsis)
The synthesis of four samples of new polyurethanes was evaluated by changing the ratio of the diol monomers used, poly(propylene glycol) (PPG) and D-isosorbide, in the presence of aliphatic isocyanates such as the isophorone diisocyanate (IPDI) and 4,4′-methylenebis(cyclohexyl isocyanate) (HMDI). The thermal properties of the four polymers obtained were determined by DSC, exhibiting Tg values in the range 55–70 °C, and their molecular structure characterized by FTIR, 1H, and 13C NMR spectroscopies. The diffusion coefficients of these polymers in solution were measured by the Pulse Gradient Spin Echo (PGSE) NMR method, enabling the calculation of the corresponding hydrodynamic radii in diluted solution (1.62–2.65 nm). The molecular weights were determined by GPC/SEC and compared with the values determined by a quantitative 13C NMR analysis. Finally, the biocompatibility of the polyurethanes was assessed using the HaCaT keratinocyte cell line by the MTT reduction assay method showing values superior to 70% cell viability. View Full-Text
Keywords: biocompatibility; GPC/SEC; keratinocyte cells; NMR; polyurethane; renewable sources biocompatibility; GPC/SEC; keratinocyte cells; NMR; polyurethane; renewable sources
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MDPI and ACS Style

Gregorí Valdés, B.S.; Gomes, C.S.B.; Gomes, P.T.; Ascenso, J.R.; Diogo, H.P.; Gonçalves, L.M.; Galhano dos Santos, R.; Ribeiro, H.M.; Bordado, J.C. Synthesis and Characterization of Isosorbide-Based Polyurethanes Exhibiting Low Cytotoxicity Towards HaCaT Human Skin Cells. Polymers 2018, 10, 1170. https://doi.org/10.3390/polym10101170

AMA Style

Gregorí Valdés BS, Gomes CSB, Gomes PT, Ascenso JR, Diogo HP, Gonçalves LM, Galhano dos Santos R, Ribeiro HM, Bordado JC. Synthesis and Characterization of Isosorbide-Based Polyurethanes Exhibiting Low Cytotoxicity Towards HaCaT Human Skin Cells. Polymers. 2018; 10(10):1170. https://doi.org/10.3390/polym10101170

Chicago/Turabian Style

Gregorí Valdés, Barbara S., Clara S.B. Gomes, Pedro T. Gomes, José R. Ascenso, Hermínio P. Diogo, Lídia M. Gonçalves, Rui Galhano dos Santos, Helena M. Ribeiro, and João C. Bordado. 2018. "Synthesis and Characterization of Isosorbide-Based Polyurethanes Exhibiting Low Cytotoxicity Towards HaCaT Human Skin Cells" Polymers 10, no. 10: 1170. https://doi.org/10.3390/polym10101170

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