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Catalysts 2019, 9(4), 317;

Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized Clostridium butyricum

Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, Slovakia
School of Chemistry and Chemical Engineering, Queen’s University Belfast, Belfast BT9 5AG, UK
Author to whom correspondence should be addressed.
Received: 31 January 2019 / Revised: 20 March 2019 / Accepted: 27 March 2019 / Published: 31 March 2019
(This article belongs to the Special Issue Biocatalysts: Design and Application)
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The present study describes the production of the value-added chemical 1,3-propanediol (1,3-PD) from crude glycerol, a waste by-product formed during biodiesel production. The efficiency, robustness, and stability of the process were improved by immobilization of the anaerobic bacterium Clostridium butyricum into a polyvinyl alcohol (PVA) hydrogel. The highest average productivity, 6.8 ± 0.2 g/(L·h), was achieved in 10 consecutive, repeated batch fermentations, with an initial concentration of pure glycerol 45.5 ± 0.7 g/L, after 2.5 h. The highest final concentration and yield of 1,3-PD, 28.3 ± 0.6 g/L, and 0.42 ± 0.01 g/g, respectively, were achieved in eleven repeated batch fermentations, after increasing the initial pure glycerol concentration to 70.4 ± 1.9 g/L. Two different types of crude glycerol, produced from used cooking oil (UCO) and rapeseed oil (RO), were tested in repeated batch fermentations, with an average productivity achieved of 2.3 ± 0.1 and 3.5 ± 0.3 g/(L·h), respectively. The highest final concentration and yield of 1,3-PD, 12.6 ± 0.9 g/L, and 0.35 ± 0.02 g/g, respectively, were observed in fifteen repeated batch fermentations with RO crude glycerol. An excellent stability of the immobilized anaerobic bacteria and increase of productivity in fermentation of crude glycerol was demonstrated. View Full-Text
Keywords: Clostridium butyricum; immobilization; 1,3-propanediol; crude glycerol Clostridium butyricum; immobilization; 1,3-propanediol; crude glycerol

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Dolejš, I.; Líšková, M.; Krasňan, V.; Markošová, K.; Rosenberg, M.; Lorenzini, F.; Marr, A.C.; Rebroš, M. Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilized Clostridium butyricum. Catalysts 2019, 9, 317.

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