An Enzyme Cascade Synthesis of Vanillin
AbstractA novel approach for the synthesis of vanillin employing a three-step two-enzymatic cascade sequence is reported. Cytochrome P450 monooxygenases are known to catalyse the selective hydroxylation of aromatic compounds, which is one of the most challenging chemical reactions. A set of rationally designed variants of CYP102A1 (P450 BM3) from Bacillus megaterium at the amino acid positions 47, 51, 87, 328 and 437 was screened for conversion of the substrate 3-methylanisole to vanillyl alcohol via the intermediate product 4-methylguaiacol. Furthermore, a vanillyl alcohol oxidase (VAO) variant (F454Y) was selected as an alternative enzyme for the transformation of one of the intermediate compounds via vanillyl alcohol to vanillin. As a proof of concept, the bi-enzymatic three-step cascade conversion of 3-methylanisole to vanillin was successfully evaluated both in vitro and in vivo. View Full-Text
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Klaus, T.; Seifert, A.; Häbe, T.; Nestl, B.M.; Hauer, B. An Enzyme Cascade Synthesis of Vanillin. Catalysts 2019, 9, 252.
Klaus T, Seifert A, Häbe T, Nestl BM, Hauer B. An Enzyme Cascade Synthesis of Vanillin. Catalysts. 2019; 9(3):252.Chicago/Turabian Style
Klaus, Tobias; Seifert, Alexander; Häbe, Tim; Nestl, Bettina M.; Hauer, Bernhard. 2019. "An Enzyme Cascade Synthesis of Vanillin." Catalysts 9, no. 3: 252.
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