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Catalysts 2019, 9(4), 361; https://doi.org/10.3390/catal9040361

Immobilization of Phospholipase D on Silica-Coated Magnetic Nanoparticles for the Synthesis of Functional Phosphatidylserine

1
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
2
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
*
Authors to whom correspondence should be addressed.
Qingqing Han and Haiyang Zhang contributed to the work equally and should be regarded as co-first authors.
Received: 22 March 2019 / Revised: 7 April 2019 / Accepted: 7 April 2019 / Published: 15 April 2019
(This article belongs to the Special Issue Biocatalysis for Industrial Applications)
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Abstract

In this study, silica-coated magnetic nanoparticles (Fe3O4/SiO2) were synthesized and applied in the immobilization of phospholipase D (PLDa2) via physical adsorption and covalent attachment. The immobilized PLDa2 was applied in the synthesis of functional phosphatidylserine (PS) through a transphophatidylation reaction. The synthesis process and characterizations of the carriers were examined by scanning electron microscope (SEM), transmission electron microscope (TEM), and Fourier-transform infrared spectroscopy (FT-IR). The optimum immobilization conditions were evaluated, and the thermal and pH stability of immobilized and free PLDa2 were measured and compared. The tolerance to high temperature of immobilized PLDa2 increased remarkably by 10°C. Furthermore, the catalytic activity of the immobilized PLDa2 remained at 40% after eight recycles, which revealed that silica-coated magnetic nanoparticles have potential application for immobilization and catalytic reactions in a biphasic system. View Full-Text
Keywords: immobilization; phospholipase D; magnetic nanoparticles; stability; phosphatidylserine immobilization; phospholipase D; magnetic nanoparticles; stability; phosphatidylserine
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Han, Q.; Zhang, H.; Sun, J.; Liu, Z.; Huang, W.-C.; Xue, C.; Mao, X. Immobilization of Phospholipase D on Silica-Coated Magnetic Nanoparticles for the Synthesis of Functional Phosphatidylserine. Catalysts 2019, 9, 361.

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