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Open AccessArticle

Surfactant Imprinting Hyperactivated Immobilized Lipase as Efficient Biocatalyst for Biodiesel Production from Waste Cooking Oil

State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China
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Catalysts 2019, 9(11), 914; https://doi.org/10.3390/catal9110914
Received: 12 October 2019 / Revised: 27 October 2019 / Accepted: 29 October 2019 / Published: 1 November 2019
(This article belongs to the Special Issue Novel Enzyme and Whole-Cell Biocatalysts)
Enzymatic production of biodiesel from waste cooking oil (WCO) could contribute to resolving the problems of energy demand and environment pollutions.In the present work, Burkholderia cepacia lipase (BCL) was activated by surfactant imprinting, and subsequently immobilized in magnetic cross-linked enzyme aggregates (mCLEAs) with hydroxyapatite coated magnetic nanoparticles (HAP-coated MNPs). The maximum hyperactivation of BCL mCLEAs was observed in the pretreatment of BCL with 0.1 mM Triton X-100. The optimized Triton-activated BCL mCLEAs was used as a highly active and robust biocatalyst for biodiesel production from WCO, exhibiting significant increase in biodiesel yield and tolerance to methanol. The results indicated that surfactant imprinting integrating mCLEAs could fix BCL in their active (open) form, experiencing a boost in activity and allowing biodiesel production performed in solvent without further addition of water. A maximal biodiesel yield of 98% was achieved under optimized conditions with molar ratio of methanol-to-WCO 7:1 in one-time addition in hexane at 40 °C. Therefore, the present study displays a versatile method for lipase immobilization and shows great practical latency in renewable biodiesel production.
Keywords: biodiesel; waste cooking oil; lipase immobilization; interfacial activation; functionalized magnetic nanoparticles biodiesel; waste cooking oil; lipase immobilization; interfacial activation; functionalized magnetic nanoparticles
MDPI and ACS Style

Yang, H.; Zhang, W. Surfactant Imprinting Hyperactivated Immobilized Lipase as Efficient Biocatalyst for Biodiesel Production from Waste Cooking Oil. Catalysts 2019, 9, 914.

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