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Open AccessReview

Cofactor F420-Dependent Enzymes: An Under-Explored Resource for Asymmetric Redox Biocatalysis

1
CSIRO Synthetic Biology Future Science Platform, Canberra 2601, Australia
2
Research School of Chemistry, Australian National University, Canberra 2601, Australia
*
Author to whom correspondence should be addressed.
M.V.S. and J.A. contributed equally to this review.
Catalysts 2019, 9(10), 868; https://doi.org/10.3390/catal9100868
Received: 20 September 2019 / Revised: 8 October 2019 / Accepted: 10 October 2019 / Published: 20 October 2019
(This article belongs to the Special Issue Novel Enzyme and Whole-Cell Biocatalysts)
The asymmetric reduction of enoates, imines and ketones are among the most important reactions in biocatalysis. These reactions are routinely conducted using enzymes that use nicotinamide cofactors as reductants. The deazaflavin cofactor F420 also has electrochemical properties that make it suitable as an alternative to nicotinamide cofactors for use in asymmetric reduction reactions. However, cofactor F420-dependent enzymes remain under-explored as a resource for biocatalysis. This review considers the cofactor F420-dependent enzyme families with the greatest potential for the discovery of new biocatalysts: the flavin/deazaflavin-dependent oxidoreductases (FDORs) and the luciferase-like hydride transferases (LLHTs). The characterized F420-dependent reductions that have the potential for adaptation for biocatalysis are discussed, and the enzymes best suited for use in the reduction of oxidized cofactor F420 to allow cofactor recycling in situ are considered. Further discussed are the recent advances in the production of cofactor F420 and its functional analog FO-5′-phosphate, which remains an impediment to the adoption of this family of enzymes for industrial biocatalytic processes. Finally, the prospects for the use of this cofactor and dependent enzymes as a resource for industrial biocatalysis are discussed. View Full-Text
Keywords: cofactor F420; deazaflavin; oxidoreductase; hydride transfer; hydrogenation; asymmetric synthesis; cofactor biosynthesis cofactor F420; deazaflavin; oxidoreductase; hydride transfer; hydrogenation; asymmetric synthesis; cofactor biosynthesis
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Shah, M.V.; Antoney, J.; Kang, S.W.; Warden, A.C.; Hartley, C.J.; Nazem-Bokaee, H.; Jackson, C.J.; Scott, C. Cofactor F420-Dependent Enzymes: An Under-Explored Resource for Asymmetric Redox Biocatalysis. Catalysts 2019, 9, 868.

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