Next Article in Journal
Multi-Catalytic Route for the Synthesis of (S)-Tembamide
Previous Article in Journal
Synthesis of Pyrazolo-Fused 4-Azafluorenones in an Ionic Liquid. Mechanistic Insights by Joint Studies Using DFT Analysis and Mass Spectrometry
Open AccessArticle

Surface Modification of Nanocrystalline TiO2 Materials with Sulfonated Porphyrins for Visible Light Antimicrobial Therapy

1
Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland
2
Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Kraków, Poland
*
Author to whom correspondence should be addressed.
Catalysts 2019, 9(10), 821; https://doi.org/10.3390/catal9100821
Received: 5 September 2019 / Revised: 24 September 2019 / Accepted: 28 September 2019 / Published: 29 September 2019
Highly-active, surface-modified anatase TiO2 nanoparticles were successfully synthesized and characterized. The morphological and optical properties of the obtained (metallo)[email protected]2 materials were evaluated using absorption and fluorescence spectroscopy, scanning electron microscopy (SEM) imaging, and dynamic light scattering (DLS). These hybrid nanoparticles efficiently generated reactive oxygen species (ROS) under blue-light irradiation (420 ± 20 nm) and possessed a unimodal size distribution of 20–70 nm in diameter. The antimicrobial performance of the synthetized agents was examined against Gram-negative and Gram-positive bacteria. After a short-term incubation of microorganisms with nanomaterials (at 1 g/L) and irradiation with blue-light at a dose of 10 J/cm2, 2–3 logs of Escherichia coli, and 3–4 logs of Staphylococcus aureus were inactivated. A further decrease in bacteria viability was observed after potentiation photodynamic inactivation (PDI), either by H2O2 or KI, resulting in complete microorganism eradication even when using low material concentration (from 0.1 g/L). SEM analysis of bacteria morphology after each mode of PDI suggested different mechanisms of cellular disruption depending on the type of generated oxygen and/or iodide species. These data suggest that TiO2-based materials modified with sulfonated porphyrins are efficient photocatalysts that could be successfully used in biomedical strategies, most notably, photodynamic inactivation of microorganisms. View Full-Text
Keywords: antimicrobial activity; photocatalysts; TiO2; nanoparticles; photosensitization; photodynamic inactivation of microorganisms (PDI) antimicrobial activity; photocatalysts; TiO2; nanoparticles; photosensitization; photodynamic inactivation of microorganisms (PDI)
Show Figures

Graphical abstract

MDPI and ACS Style

Sułek, A.; Pucelik, B.; Kobielusz, M.; Łabuz, P.; Dubin, G.; Dąbrowski, J.M. Surface Modification of Nanocrystalline TiO2 Materials with Sulfonated Porphyrins for Visible Light Antimicrobial Therapy. Catalysts 2019, 9, 821.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop