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Open AccessArticle

Conversion of Shrimp Head Waste for Production of a Thermotolerant, Detergent-Stable, Alkaline Protease by Paenibacillus sp.

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Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan
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Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam
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Doctoral Program in Applied Sciences, College of Science, Tamkang University, New Taipei City 25137, Taiwan
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Department of Chemical and Materials Engineering, Tamkang University, New Taipei City 25137, Taiwan
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Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam
6
Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan
*
Author to whom correspondence should be addressed.
Catalysts 2019, 9(10), 798; https://doi.org/10.3390/catal9100798
Received: 14 August 2019 / Revised: 6 September 2019 / Accepted: 23 September 2019 / Published: 24 September 2019
(This article belongs to the Special Issue Biocatalytic Applications in Biotechnology)
Fishery processing by-products have been of great interest to researchers due to their beneficial applications in many fields. In this study, five types of marine by-products, including demineralized crab shell, demineralized shrimp shell, shrimp head, shrimp shell, and squid pen, provided sources of carbon and nitrogen nutrition by producing a protease from Paenibacillus sp. TKU047. Strain TKU047 demonstrated the highest protease productivity (2.98 U/mL) when cultured for two days on a medium containing 0.5% of shrimp head powder (SHP). The mass of TKU047 protease was determined to be 32 kDa (approximately). TKU047 protease displayed optimal activity at 70–80 °C and pH 9, with a pH range of stability from 6 to 11. TKU047 protease also showed stability in solutions containing surfactants and detergents. Based on its excellent properties, Paenibacillus sp. TKU047 protease may be a feasible candidate for inclusion in laundry detergents. View Full-Text
Keywords: marine chitinous by-products; Paenibacillus; protease; shrimp heads marine chitinous by-products; Paenibacillus; protease; shrimp heads
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MDPI and ACS Style

Doan, C.T.; Tran, T.N.; Wen, I.-H.; Nguyen, V.B.; Nguyen, A.D.; Wang, S.-L. Conversion of Shrimp Head Waste for Production of a Thermotolerant, Detergent-Stable, Alkaline Protease by Paenibacillus sp.. Catalysts 2019, 9, 798.

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