Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin

Yang, Chengyu; Fan, Weiming; Zhang, Ruijie; Shi, Jiping; Knežević-Jugović, Zorica; Zhang, Baoguo

doi:10.3390/catal9010005

Open AccessArticle

Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin

by

Chengyu Yang

^1,2,

Weiming Fan

^2,3,

Ruijie Zhang

^1,2,4,

Jiping Shi

^1,2,4,*,

Zorica Knežević-Jugović

⁵ and

Baoguo Zhang

^1,2,*

¹

Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai 201210, China

²

University of Chinese Academy of Sciences, Beijing 100049, China

³

Zhejiang Zhenyuan Pharmaceutical Co., Ltd. No. 61 North Yuedong Road, Shaoxing 312000, China

⁴

School of Life Science and Technology, Shanghai Tech University, No. 393 Huaxia Middle Road, Pudong, Shanghai 201210, China

⁵

Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade 11000, Serbia

^*

Authors to whom correspondence should be addressed.

Catalysts 2019, 9(1), 5; https://doi.org/10.3390/catal9010005

Submission received: 5 December 2018 / Revised: 16 December 2018 / Accepted: 18 December 2018 / Published: 21 December 2018

(This article belongs to the Special Issue Biocatalysts: Design and Application)

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Abstract

α-Arbutin (4-hydroquinone-α-D-glucopyranoside), an effective skin-lightening agent due to its considerable inhibitory effect on human tyrosinase activity, is widely used in the pharmaceutical and cosmetic industries. Recently, α-arbutin was prepared through transglucosylation of hydroquinone using microbial glycosyltransferases as catalysts. However, the low yield and prolonged reaction time of the biotransformation process of α-arbutin production limited its industrial application. In this work, an amylosucrase (ASase) from Xanthomonas campestris pv. campestris str. ATCC 33913 (XcAS) was expressed efficiently in Escherichia coli JM109. The catalytic property of the purified XcAS for the synthesis of α-arbutin was tested. The recombinant strain was applied for highly efficient synthesis of α-arbutin using sucrose and hydroquinone as glucosyl donor and acceptor, respectively. By optimizing the biotransformation conditions and applying a fed-batch strategy, the final production yield and conversion rate of α-arbutin reached 60.9 g/L and 95.5%, respectively, which is the highest reported yield by engineered strains. Compared to the highest reported value (<1.4 g/L/h), our productivity (7.6 g/L/h) was improved more than five-fold. This work represents an efficient and rapid method for α-arbutin production with potential industrial applications.

Keywords: Amylosucrase; Xanthomonas campestris pv. Campestris; α-arbutin; enzymatic properties; whole-cell biocatalyst; fed-batch strategy

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MDPI and ACS Style

Yang, C.; Fan, W.; Zhang, R.; Shi, J.; Knežević-Jugović, Z.; Zhang, B. Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin. Catalysts 2019, 9, 5. https://doi.org/10.3390/catal9010005

AMA Style

Yang C, Fan W, Zhang R, Shi J, Knežević-Jugović Z, Zhang B. Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin. Catalysts. 2019; 9(1):5. https://doi.org/10.3390/catal9010005

Chicago/Turabian Style

Yang, Chengyu, Weiming Fan, Ruijie Zhang, Jiping Shi, Zorica Knežević-Jugović, and Baoguo Zhang. 2019. "Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin" Catalysts 9, no. 1: 5. https://doi.org/10.3390/catal9010005

APA Style

Yang, C., Fan, W., Zhang, R., Shi, J., Knežević-Jugović, Z., & Zhang, B. (2019). Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin. Catalysts, 9(1), 5. https://doi.org/10.3390/catal9010005

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Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of α-Arbutin

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