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Sequential Biotransformation of Antcin K by Bacillus subtilis ATCC 6633

Department of Biological Sciences and Technology, National University of Tainan, No. 33, Sec. 2, Shu-Lin St., Tainan 70005, Taiwan
Department of Biotechnology, Chia Nan University of Pharmacy and Science, No. 60, Sec. 1, Erh-Jen Rd., Jen-Te District, Tainan 71710, Taiwan
Department of Food Science, National Quemoy University, No. 1, University Rd., Jin-Ning Township, Kinmen County 892, Taiwan
Author to whom correspondence should be addressed.
Catalysts 2018, 8(9), 349;
Received: 6 August 2018 / Revised: 19 August 2018 / Accepted: 23 August 2018 / Published: 27 August 2018
(This article belongs to the Special Issue Novel Enzyme and Whole-Cell Biocatalysis)
PDF [3054 KB, uploaded 27 August 2018]


The biotransformation of antcin K, a major ergostane triterpenoid from the fruiting bodies of Antrodia cinnamomea, by Bacillus subtilis (B. subtilis) ATCC 6633 was studied. Four metabolites from the biotransformation were isolated with preparative high-performance liquid chromatography and identified as 25S-antcin K 26-O-β-glucoside, 25R-antcin K 26-O-β-glucoside, 25S-antcin K 26-O-β-(6′-O-succinyl)-glucoside, and 25R-antcin K 26-O-β-(6′-O-succinyl)-glucoside with mass and nuclear magnetic resonance spectral analysis. By using either 25S-antcin K 26-O-β-glucoside or 25R-antcin K 26-O-β-glucoside as the biotransformation precursor, it was proven that 25S-antcin K 26-O-β-(6′-O-succinyl)-glucoside and 25R-antcin K 26-O-β-(6′-O-succinyl)-glucoside were biotransformed from 25S-antcin K 26-O-β-glucoside and 25R-antcin K 26-O-β-glucoside, respectively. To the best of our knowledge, this is the first study on the glycosylation of triterpenoids from A. cinnamomea, and the first time the succinylation of triterpenoid glycosides by microorganisms has been found. In addition, all four antcin K glucoside derivatives are new compounds. View Full-Text
Keywords: Antrodia cinnamomea; antcin K; Bacillus subtilis; biotransformation; glycosylation Antrodia cinnamomea; antcin K; Bacillus subtilis; biotransformation; glycosylation

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Chang, T.-S.; Chiang, C.-M.; Siao, Y.-Y.; Wu, J.-Y. Sequential Biotransformation of Antcin K by Bacillus subtilis ATCC 6633. Catalysts 2018, 8, 349.

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