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Open AccessArticle

The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties

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Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-97187 Luleå, Sweden
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Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
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Dupont Industrial Biosciences, Nieuwe Kanaal 7-S, 6709 PA Wageningen, The Netherlands
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Department of Microbiology, University of Helsinki, Viikinkaari 9, 00014-FIN Helsinki, Finland
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Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo Via Cintia, 4 IT-80126 Naples, Italy
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Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-41296 Gothenburg, Sweden
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Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstraße 3, 48149 Münster, Germany
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Taros Chemicals GmbH & Co KG, Emil-Figge-Str. 76a, 44227 Dortmund, Germany
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Environmental Sciences Department, King Abdulaziz University, Jeddah 21589, Saudi Arabia
*
Author to whom correspondence should be addressed.
Catalysts 2018, 8(6), 242; https://doi.org/10.3390/catal8060242
Received: 21 May 2018 / Revised: 31 May 2018 / Accepted: 1 June 2018 / Published: 7 June 2018
(This article belongs to the Special Issue Novel Enzyme and Whole-Cell Biocatalysis)
Twenty-eight fungal feruloyl esterases (FAEs) were evaluated for their synthetic abilities in a ternary system of n-hexane: t-butanol: 100 mM MOPS-NaOH pH 6.0 forming detergentless microemulsions. Five main derivatives were synthesized, namely prenyl ferulate, prenyl caffeate, butyl ferulate, glyceryl ferulate, and l-arabinose ferulate, offering, in general, higher yields when more hydrophilic alcohol substitutions were used. Acetyl xylan esterase-related FAEs belonging to phylogenetic subfamilies (SF) 5 and 6 showed increased synthetic yields among tested enzymes. In particular, it was shown that FAEs belonging to SF6 generally transesterified aliphatic alcohols more efficiently while SF5 members preferred bulkier l-arabinose. Predicted surface properties and structural characteristics were correlated with the synthetic potential of selected tannase-related, acetyl-xylan-related, and lipase-related FAEs (SF1-2, -6, -7 members) based on homology modeling and small molecular docking simulations. View Full-Text
Keywords: transesterification; feruloyl esterase; antioxidants; phylogenetic classification; prenyl ferulate; prenyl caffeate; glyceryl ferulate; butyl ferulate; l-arabinose ferulate; sugar esters transesterification; feruloyl esterase; antioxidants; phylogenetic classification; prenyl ferulate; prenyl caffeate; glyceryl ferulate; butyl ferulate; l-arabinose ferulate; sugar esters
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Antonopoulou, I.; Dilokpimol, A.; Iancu, L.; Mäkelä, M.R.; Varriale, S.; Cerullo, G.; Hüttner, S.; Uthoff, S.; Jütten, P.; Piechot, A.; Steinbüchel, A.; Olsson, L.; Faraco, V.; Hildén, K.S.; De Vries, R.P.; Rova, U.; Christakopoulos, P. The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties. Catalysts 2018, 8, 242.

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