A Combined Computational–Experimental Study on the Substrate Binding and Reaction Mechanism of Salicylic Acid Decarboxylase
Abstract
:1. Introduction
2. Results and Discussion
3. Computational and Experimental Details
3.1. Computational Details
3.2. Active Site Model
3.3. Cloning and Protein Purification
3.4. Crystallization, Data Collection, Structure Determination, and Refinement
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chen, F.; Zhao, Y.; Zhang, C.; Wang, W.; Gao, J.; Li, Q.; Qin, H.; Dai, Y.; Liu, W.; Liu, F.; et al. A Combined Computational–Experimental Study on the Substrate Binding and Reaction Mechanism of Salicylic Acid Decarboxylase. Catalysts 2022, 12, 1577. https://doi.org/10.3390/catal12121577
Chen F, Zhao Y, Zhang C, Wang W, Gao J, Li Q, Qin H, Dai Y, Liu W, Liu F, et al. A Combined Computational–Experimental Study on the Substrate Binding and Reaction Mechanism of Salicylic Acid Decarboxylase. Catalysts. 2022; 12(12):1577. https://doi.org/10.3390/catal12121577
Chicago/Turabian StyleChen, Fuqiang, Yipei Zhao, Chenghua Zhang, Wei Wang, Jian Gao, Qian Li, Huimin Qin, Yujie Dai, Weidong Liu, Fufeng Liu, and et al. 2022. "A Combined Computational–Experimental Study on the Substrate Binding and Reaction Mechanism of Salicylic Acid Decarboxylase" Catalysts 12, no. 12: 1577. https://doi.org/10.3390/catal12121577