Direct Electron Transfer-Type Bioelectrocatalysis of Redox Enzymes at Nanostructured Electrodes
Round 1
Reviewer 1 Report
The authors presented a review on the recent advances regarding the direct electron transfer-type bioelectrocatalysis, which couples electrode reactions and catalytic functions of redox enzymes without any redox mediator. This manuscript shows a well-organized work, the results from the available literature being comprehensively described. There are some aspects that must be improved before recommending its publication in Catalysts.
- I suggest to reduce the number of keywords.
- The English language should be strongly revised throughout all Abstract & Manuscript (some examples can be in line 16 – recent, line 18 – There are described basic, line 78 – against that of, line 483 – for different applications).
- Please adjust the font of the figure captions according to the guidelines.
- What is the yellow line in the Figure 4? Please check the image accuracy.
- You should explain the significance of T1 and T2/3 in the legend of Figure 6.
- I suggest to put the enzymes in sub-sub-sections.
- Please provide references for sentence in lines 153-154.
Author Response
Reply to reviewer 1:
We appreciate for the comments from the reviewer. We have revised our manuscript along the lines suggested by the reviewer. The revision and replies are detailed in the following.
Reply to comment 1:
According to the comment, some keywords “bioelectrocatalysis (duplication); hydrogenase; fructose dehydrogenase; bilirubin oxidase; formate dehydrogenase; ferredoxin-NADP+ reductase” have been removed.
Reply to comment 2:
Thank you very much for the kind comments from the reviewer. Many parts including lines 16, 18, 78, and 483 were corrected.
Reply to comment 3:
We used a template of this journal and adjust the font.
Reply to comment 4:
We are sorry. We could not find any yellow line in the figure.
Reply to comment 5:
As suggested by the reviewer, the site of the reduction of oxygen and the reaction site with the electrode were added in Fig. 6.
Reply to comment 6:
We prepared it by use of a template and the enzyme sections were put in sub-(sub-)sections.
Reply to comment 7:
The corresponding references have been cited in the following individual sections (issues).
We hope the problems in our MS now have been satisfactorily addressed.
Reviewer 2 Report
The review presented by Kano and his coauthors bring together enlightening examples on bioelectrocatalytic systems focused on direct electron transfer, covering both theoretical aspects and providing plenty examples on successful bioelectrode configurations. I am glad to support its publication after considering a couple of minor upgrades:
Regarding nanostructured carbon-made electrodes for hydrogenases, a very interesting work where carbon nanotubes were implemented on graphite electrodes to be used as scaffold for covalently-attached DET hydrogenase should be included. This remarkable work managed to work at least during four weeks in non-stop conditions (Nano Letters 2007, 7, 6, 1603-1608).
The authors are reviewing the activity of bilirubin oxidase on gold nanoparticle-modified electrodes (lines 302-307), saying that the size of the particles ranging from 20 to 80 nm does not make a difference. Any of these particles are much larger than BOD, sized approximately at 5 nm3. If the authors want to report on gold nanoparticles making a difference on multicopper oxidases there is a work by Shleev and collaborators where Trametes hirsuta laccase shows an electron transfer dependance upon different nanoparticle sizes, using 5 nm and 16 nm in diameter gold nanoparticles (J. Am. Chem. Soc. 2012, 134, 17212-17220 DOI: 10.1021/ja307308j).
Author Response
Reply to reviewer 2:
We appreciate for the comments from the reviewer. We have revised our manuscript along the lines suggested by the reviewer. The revision and replies are detailed in the following.
Reply to comment 1:
The paper was added as ref. 43 in the manuscript.
Reply to comment 2:
The paper recommended by the reviewer was added as ref. 49 in the manuscript.
We hope the problems in our MS now have been satisfactorily addressed.
