The Matrix Stiffness Coordinates the Cell Proliferation and PD-L1 Expression via YAP in Lung Adenocarcinoma
Abstract
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Transient Transfection of Cell Lines
2.2. Polyacrylamide Hydrogel
2.3. Spheroid 3D Culture
2.4. Western Blotting Assay
2.5. siRNA-Mediated Gene Expression Knockdown
2.6. Immunofluorescence
2.7. RT-PCR
3. Results
3.1. Matrix Stiffness Regulates the Cell Proliferation and the Expiration of Both YAP and PD-L1 in Lung Adenocarcinoma
3.2. YAP Plays an Important Role in Regulating the PD-L1 Expression and Proliferating Capacity in the Stiff Matrix
3.3. YAP Regulates the Expression of PD-L1 and Cell Proliferation in 3D Culture Conditions
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Park, Y.; Lee, D.; Lee, J.E.; Park, H.S.; Jung, S.S.; Park, D.; Kang, D.H.; Lee, S.-I.; Woo, S.-D.; Chung, C. The Matrix Stiffness Coordinates the Cell Proliferation and PD-L1 Expression via YAP in Lung Adenocarcinoma. Cancers 2024, 16, 598. https://doi.org/10.3390/cancers16030598
Park Y, Lee D, Lee JE, Park HS, Jung SS, Park D, Kang DH, Lee S-I, Woo S-D, Chung C. The Matrix Stiffness Coordinates the Cell Proliferation and PD-L1 Expression via YAP in Lung Adenocarcinoma. Cancers. 2024; 16(3):598. https://doi.org/10.3390/cancers16030598
Chicago/Turabian StylePark, Yeonhee, Dahye Lee, Jeong Eun Lee, Hee Sun Park, Sung Soo Jung, Dongil Park, Da Hyun Kang, Song-I Lee, Seong-Dae Woo, and Chaeuk Chung. 2024. "The Matrix Stiffness Coordinates the Cell Proliferation and PD-L1 Expression via YAP in Lung Adenocarcinoma" Cancers 16, no. 3: 598. https://doi.org/10.3390/cancers16030598
APA StylePark, Y., Lee, D., Lee, J. E., Park, H. S., Jung, S. S., Park, D., Kang, D. H., Lee, S.-I., Woo, S.-D., & Chung, C. (2024). The Matrix Stiffness Coordinates the Cell Proliferation and PD-L1 Expression via YAP in Lung Adenocarcinoma. Cancers, 16(3), 598. https://doi.org/10.3390/cancers16030598