ID2 Promotes Lineage Transition of Prostate Cancer through FGFR and JAK-STAT Signaling
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. ID2 Expression Is Upregulated in NEPC and DNPC
2.2. Transcriptomic Reprogramming and Pathway Analysis upon ID2 Overexpression
2.3. ID2 Attenuates AR Signaling and Promotes NETD
2.4. ID2 Enhances PCa Cell Aggressiveness
2.5. ID2 UP50 Signature Generation and Validation of Its Effectiveness
2.6. ID2 Activates FGFR Signaling and JAK-STAT Signaling to Promote Lineage Transition
3. Discussion
4. Methods
4.1. Cell Culture
4.2. Animal Studies
4.3. Western Blot
4.4. Cell Proliferation Assay
4.5. Cell Invasion Assay
4.6. Cell Migration Assay
4.7. RNA-Seq and Data Analysis
4.8. Signature Score Calculation
4.9. Clinical Data Analysis
4.10. qRT-PCR
4.11. Overexpression
4.12. shRNA Knockdown
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, J.; Chen, Z.; Mao, Y.; He, Y.; Wu, X.; Wu, J.; Sheng, L. ID2 Promotes Lineage Transition of Prostate Cancer through FGFR and JAK-STAT Signaling. Cancers 2024, 16, 392. https://doi.org/10.3390/cancers16020392
Zhang J, Chen Z, Mao Y, He Y, Wu X, Wu J, Sheng L. ID2 Promotes Lineage Transition of Prostate Cancer through FGFR and JAK-STAT Signaling. Cancers. 2024; 16(2):392. https://doi.org/10.3390/cancers16020392
Chicago/Turabian StyleZhang, Jinxiong, Zhihao Chen, Yongxin Mao, Yijun He, Xin Wu, Jianhong Wu, and Lu Sheng. 2024. "ID2 Promotes Lineage Transition of Prostate Cancer through FGFR and JAK-STAT Signaling" Cancers 16, no. 2: 392. https://doi.org/10.3390/cancers16020392