Distribution of HPV Subtypes in Diverse Anogenital and Oral Samples from Women and Correlation of Infections with Neoplasia of the Cervix
by
, ,
Karen Bräutigam
1,*
,
Stefanie Meier
1,
Sabina Meneder
1,
Louisa Proppe
1,
Katharina Stroschein
1,
Stephan Polack
1,
Frank Köster
1,
Achim Rody
1 and
Sascha Baum
2 1
Department of Gynecology and Obstetrics, Campus Lübeck, University Medical Center Schleswig-Holstein, 23538 Lübeck, Germany
2
Gynäkologie des MVZ Westpfalz, 67655 Kaiserslautern, Germany
*
Author to whom correspondence should be addressed.
Cancers 2022, 14(13), 3136; https://doi.org/10.3390/cancers14133136
Submission received: 9 June 2022
/
Revised: 22 June 2022
/
Accepted: 24 June 2022
/
Published: 26 June 2022
(This article belongs to the Special Issue Biological, Clinical and Epidemiological Features of Human Papillomavirus-Associated Cancers)
Simple Summary
Human papilloma virus (HPV)-associated cancers and their precursors are increasing worldwide. The purpose of our study was to investigate HPV subtypes in anogenital and oral samples to analyze the distribution and frequency of high- and low-risk HPV in the cervix, vagina, vulva, anus, and oral cavity. Identification of special HPV subtypes for these areas may help to increase the prognostic value of HPV screening for early detection of precursors or cancers being highly HPV-attributable. HPV genotyping with the EUROArray HPV test was performed in 509 patient samples from our dysplasia consultation. In addition to the well described HPV, e.g., HPV 16 and HPV 31, we detected further HPV subtypes with higher incidences in the investigated areas (e.g., HPV 53 and HPV 73) which may be evident for precursors and cancers of all areas.
Abstract
Background: Cancers and intraepithelial lesions of different anogenital areas as well as oral cancer are associated with human papilloma virus (HPV) infections. Methods: In this study cervical, vaginal, vulvar, anal, and oral samples were taken from 509 patients visiting our dysplasia consultation clinic. HPV genotyping was performed using the EUROArray HPV test. Results: Positivity of HR HPV was found in 60.4–64.3% of anogenital and 14.6% of oral samples. HPV 16 showed the highest incidence in all investigated areas. In cervical and vaginal samples HPV 31 was detected second most, while in vulvar, anal, and oral samples HPV 53 was the second most common subtype. HPV 18 was found lower in all areas, while HPV 51, HPV 52, and HPV 73 were detected higher than expected from published data. A good concordance between cervical, vaginal and vulvar samples was examined for most of the HPV. HR HPV infection was higher in cervical cancer (CC; 91.7%) and high-grade intraepithelial squamous lesions (HSIL; 93.9%) compared to low-grade SIL (LSIL; 69.6%) and normal samples (44.8%). Conclusion: In addition to the well described HPV subtypes, we found others with high incidences in the investigated areas which may be evident for HSIL and CC of those areas.
