EGFRvIII Promotes Cell Survival during Endoplasmic Reticulum Stress through a Reticulocalbin 1-Dependent Mechanism
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. RCN1 Expression Correlates with Glioma Grade and Glioblastoma Patient Survival
2.2. RCN1 Is Highly Expressed in EGFRvIII Positive Glioblastoma Cells
2.3. RCN1 Expression Correlates with Increased Survival after the Initiation of ER Stress
2.4. Knockdown of RCN1 Significantly Decreases Cell Survival and RCN1 Over-Expression Significantly Increases Cell Survival Following the Initiation of ER Stress
2.5. RCN1 Expression Reduces ATF6 Activity When Challenged with Tunicamycin and Thapsigargin
2.6. RCN1 Expression Protects Cells from ER Stress-Mediated Apoptosis
3. Discussion
4. Materials and Methods
4.1. Antibodies and Reagents
4.2. Cell Culture
4.3. Generation of Cells with Varying Levels of EGFR and EGFRvIII
4.4. Generation of Cells with RCN1 Over-Expression and siRNA Transfection
4.5. Cell Viability Assays
4.6. Western Blotting and Immunoprecipitation
4.7. RNA Extraction and RT-PCR
4.8. ATF6 and ARE-Luc Luciferase Assay
4.9. Caspase3/7 Assay
4.10. OncoLnc (TCGA)
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Gomez, J.; Areeb, Z.; Stuart, S.F.; Nguyen, H.P.T.; Paradiso, L.; Zulkifli, A.; Madan, S.; Rajagopal, V.; Montgomery, M.K.; Gan, H.K.; et al. EGFRvIII Promotes Cell Survival during Endoplasmic Reticulum Stress through a Reticulocalbin 1-Dependent Mechanism. Cancers 2021, 13, 1198. https://doi.org/10.3390/cancers13061198
Gomez J, Areeb Z, Stuart SF, Nguyen HPT, Paradiso L, Zulkifli A, Madan S, Rajagopal V, Montgomery MK, Gan HK, et al. EGFRvIII Promotes Cell Survival during Endoplasmic Reticulum Stress through a Reticulocalbin 1-Dependent Mechanism. Cancers. 2021; 13(6):1198. https://doi.org/10.3390/cancers13061198
Chicago/Turabian StyleGomez, Juliana, Zammam Areeb, Sarah F. Stuart, Hong P. T. Nguyen, Lucia Paradiso, Ahmad Zulkifli, Sonakshi Madan, Vijay Rajagopal, Magdalene K. Montgomery, Hui K. Gan, and et al. 2021. "EGFRvIII Promotes Cell Survival during Endoplasmic Reticulum Stress through a Reticulocalbin 1-Dependent Mechanism" Cancers 13, no. 6: 1198. https://doi.org/10.3390/cancers13061198