RIP140 Represses Intestinal Paneth Cell Differentiation and Interplays with SOX9 Signaling in Colorectal Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Organoids
2.3. Real-Time Quantitative PCR (RT-qPCR)
2.4. Histological and Immunostaining Analysis
2.5. Cell Culture and Transfections
2.6. GST Pull-Down Assay
2.7. DuoLink Proximity Ligation Assay
2.8. DNA Microarray and RNA Sequencing Analysis
2.9. Statistical Analysis
3. Results
3.1. Rip140 Inhibits Sox9 Expression and Paneth Cell Lineage in Mouse Intestine
3.2. Repression of Sox9 Expression and Paneth Cell Differentiation by Rip140 in Organoid Culture
3.3. RIP140 Is Transcriptionally Repressed by the Wnt Pathway in Human Colorectal Cell Lines
3.4. RIP140 Interacts with SOX9 and Represses Its Activity
3.5. RIP140 and SOX9 Are Opposite Prognosis Markers in CRC
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gleizes, A.; Triki, M.; Bonnet, S.; Baccari, N.; Jimenez-Dominguez, G.; Covinhes, A.; Pirot, N.; Blache, P.; Yuan, R.; Győrffy, B.; et al. RIP140 Represses Intestinal Paneth Cell Differentiation and Interplays with SOX9 Signaling in Colorectal Cancer. Cancers 2021, 13, 3192. https://doi.org/10.3390/cancers13133192
Gleizes A, Triki M, Bonnet S, Baccari N, Jimenez-Dominguez G, Covinhes A, Pirot N, Blache P, Yuan R, Győrffy B, et al. RIP140 Represses Intestinal Paneth Cell Differentiation and Interplays with SOX9 Signaling in Colorectal Cancer. Cancers. 2021; 13(13):3192. https://doi.org/10.3390/cancers13133192
Chicago/Turabian StyleGleizes, Antoine, Mouna Triki, Sandrine Bonnet, Naomi Baccari, Gabriel Jimenez-Dominguez, Aurélie Covinhes, Nelly Pirot, Philippe Blache, Rong Yuan, Balázs Győrffy, and et al. 2021. "RIP140 Represses Intestinal Paneth Cell Differentiation and Interplays with SOX9 Signaling in Colorectal Cancer" Cancers 13, no. 13: 3192. https://doi.org/10.3390/cancers13133192